Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation

doi:10.1038/srep19245

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation
	Wu, Jie ; Wang, Xiao-Chen ; Liu, Yang ; Du, Hui ; Shu, Qing-Yan ; Su, Shang ; Wang, Li-Jin ; Li, Shan-Shan ; Wang, Liang-Sheng
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep19245
文献子类	Article
英文摘要	Flavones are important secondary metabolites found in many plants. In Lonicera species, flavones contribute both physiological and pharmaceutical properties. However, flavone synthase (FNS), the key enzyme responsible for flavone biosynthesis, has not yet been characterized in Lonicera species. In this study, FNSII genes were identified from Lonicera japonica Thunb. and L. macranthoides Hand. Mazz. In the presence of NADPH, the recombinant cytochrome P450 proteins encoded by LjFNSII-1.1, LjFNSII-2.1, and LmFNSII-1.1 converted eriodictyol, naringenin, and liquiritigenin to the corresponding flavones directly. The different catalytic properties between LjFNSII-2.1 and LjFNSII-1.1 were caused by a single amino acid substitution at position 242 (glutamic acid to lysine). A methionine at position 206 and a leucine at position 381 contributed considerably to the high catalytic activity of LjFNSII-1.1. In addition, LjFNSII-1.1&2.1 and LmFNSII-1.1 also biosynthesize flavones that were further modified by O-glycosylation in transgenic tobacco. The expression levels of the FNSII genes were consistent with flavone accumulation patterns in flower buds. Our findings suggested that the weak catalytic activity of LmFNSII-1.1 and the relatively low expression of LmFNSII-1.1 in flowers might be responsible for the low levels of flavone accumulation in flower buds of L. macranthoides.
学科主题	Multidisciplinary Sciences
出版地	LONDON
WOS关键词	CYTOCHROME-P450 GENES ; INDUCIBLE FLAVONE ; IDENTIFICATION ; EXPRESSION ; BIOSYNTHESIS ; CLONING ; MAIZE ; GLYCOSYLATION ; 2-HYDROXYLASE ; LUTEOLIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000368108000002
资助机构	National Science and Technology Basic Project of China [2014FY110100]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25385]
专题	中科院植物分子生理学重点实验室
作者单位	1.Wang, Li-Jin 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Wang, Xiao-Chen 5.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China 6.Li, Shan-Shan 7.Su, Shang 8.Shu, Qing-Yan 9.Du, Hui 10.Liu, Yang
推荐引用方式 GB/T 7714	Wu, Jie,Wang, Xiao-Chen,Liu, Yang,et al. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation[J]. SCIENTIFIC REPORTS,2016,6.
APA	Wu, Jie.,Wang, Xiao-Chen.,Liu, Yang.,Du, Hui.,Shu, Qing-Yan.,...&Wang, Liang-Sheng.(2016).Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation.SCIENTIFIC REPORTS,6.
MLA	Wu, Jie,et al."Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation".SCIENTIFIC REPORTS 6(2016).