Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii

doi:10.1111/tpj.13133

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii
	Yang, Hao 1; Yang, Ning ; Wang, Tai
刊名	PLANT JOURNAL
	2016
卷号	85 期号:5 页码:660-674
关键词	histone program histone variant male germline sperm cells generative cells vegetative cells Lilium davidii
ISSN号	0960-7412
DOI	10.1111/tpj.13133
文献子类	Article
英文摘要	In flowering plants, male germline fate is determined after asymmetric division of the haploid microspore. Daughter cells have distinct fates: the generative cell (GC) undergoes further mitosis to generate sperm cells (SCs), and the vegetative cell (VC) terminally differentiates. However, our understanding of the mechanisms underlying germline development remains limited. Histone variants and modifications define chromatin states, and contribute to establishing and maintaining cell identities by affecting gene expression. Here, we constructed a lily protein database, then extracted and detailed histone entries into a comprehensive lily histone database. We isolated large amounts of nuclei from VCs, GCs and SCs from lily, and profiled histone variants of all five histone families in all three cell types using proteomics approaches. We revealed 92 identities representing 32 histone variants: six for H1, 11 for H2A, eight for H2B, five for H3 and two for H4. Nine variants, including five H1, two H2B, one H3 and one H4 variant, specifically accumulated in GCs and SCs. We also detected H3 modification patterns in the three cell types. GCs and SCs had almost identical histone profiles and similar H3 modification patterns, which were significantly different from those of VCs. Our study also revealed the presence of multiple isoforms, and differential expression patterns between isoforms of a variant. The results suggest that differential histone programs between the germline and companion VCs may be established following the asymmetric division, and are important for identity establishment and differentiation of the male germline as well as the VC. Significance Statement Histone variants and modifications define chromatin states and contribute to establishing and maintaining cell identities by affecting gene expression. Using proteomics of lily pollen, we show that there are distinct histone profiles in the vegetative cell and in the generative cell and its progeny sperm cells. We propose that differential histone programmes are important for identity establishment and differentiation of the male germline and the vegetative cell.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	MALE GAMETOPHYTE DEVELOPMENT ; MALE GAMETIC CELLS ; MASS-SPECTROMETRY ; SPERM CELLS ; GENERATIVE CELLS ; POSTTRANSLATIONAL MODIFICATIONS ; CONSTITUTIVE HETEROCHROMATIN ; QUANTITATIVE PROTEOMICS ; CHROMATIN MODIFICATIONS ; ARABIDOPSIS-THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000372274500007
资助机构	Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB910504, 2013CB945101]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25361]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Hao,Yang, Ning,Wang, Tai. Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii[J]. PLANT JOURNAL,2016,85(5):660-674.
APA	Yang, Hao,Yang, Ning,&Wang, Tai.(2016).Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii.PLANT JOURNAL,85(5),660-674.
MLA	Yang, Hao,et al."Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii".PLANT JOURNAL 85.5(2016):660-674.