Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust

doi:10.1038/srep25510

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	Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust
	An, Tianyue 2; Cai, Yanli 2; Zhao, Suzhen ; Zhou, Jianghong 1; Song, Bo ; Bux, Hadi 3; Qi, Xiaoquan
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep25510
文献子类	Article
英文摘要	Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases and can cause severe yield losses in many regions of the world. Because of the large size and complexity of wheat genome, it is difficult to study the molecular mechanism of interaction between wheat and PST. Brachypodium distachyon has become a model system for temperate grasses' functional genomics research. The phenotypic evaluation showed that the response of Brachypodium distachyon to PST was nonhost resistance (NHR), which allowed us to present this plant-pathogen system as a model to explore the immune response and the molecular mechanism underlying wheat and PST. Here we reported the generation of about 7,000 T-DNA insertion lines based on a highly efficient Agrobacterium-mediated transformation system. Hundreds of mutants either more susceptible or more resistant to PST than that of the wild type Bd21 were obtained. The three putative target genes, Bradi5g17540, BdMYB102 and Bradi5g11590, of three T-DNA insertion mutants could be involved in NHR of Brachypodium distachyon to wheat stripe rust. The systemic pathologic study of this T-DNA mutants would broaden our knowledge of NHR, and assist in breeding wheat cultivars with durable resistance.
学科主题	Multidisciplinary Sciences
出版地	BERLIN
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; ARABIDOPSIS-THALIANA ; DURABLE RESISTANCE ; DEFENSE RESPONSES ; CONTRIBUTES ; INFECTION ; SYSTEM ; HOST ; PATHOGENS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PORTFOLIO
WOS记录号	WOS:000375254600001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471756, 31270413]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25338]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Sindh, Inst Plant Sci, Jamshoro 76080, Pakistan 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 4.Beijing Inst Landscape Architecture, Beijing 100102, Peoples R China
推荐引用方式 GB/T 7714	An, Tianyue,Cai, Yanli,Zhao, Suzhen,et al. Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust[J]. SCIENTIFIC REPORTS,2016,6.
APA	An, Tianyue.,Cai, Yanli.,Zhao, Suzhen.,Zhou, Jianghong.,Song, Bo.,...&Qi, Xiaoquan.(2016).Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust.SCIENTIFIC REPORTS,6.
MLA	An, Tianyue,et al."Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust".SCIENTIFIC REPORTS 6(2016).