The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species

doi:10.1093/pcp/pcw006

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	The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species
	Qi, Shilian 1,3; Lin, Qingfang 1,3; Zhu, Huishan 3; Gao, Fenghua 3; Zhang, Wenhao 2; Hua, Xuejun 3
刊名	PLANT AND CELL PHYSIOLOGY
	2016
卷号	57 期号:3 页码:528-539
关键词	E3 Ligase RING-H2 salt tolerance Solanum pimpinellifolium VIGS
ISSN号	0032-0781
DOI	10.1093/pcp/pcw006
文献子类	Article
英文摘要	Protein ubiquitination in plants plays critical roles in many biological processes, including adaptation to abiotic stresses. Previously, RING finger E3 ligase has been characterized during salt stress response in several plant species, but little is known about its function in tomato. Here, we report that SpRing, a stress-inducible gene, is involved in salt stress signaling in wild tomato species Solanum pimpinellifolium 'PI365967'. In vitro ubiquitination assay revealed that SpRing is an E3 ubiquitin ligase and the RING finger conserved region is required for its activity. SpRing is expressed in all tissues of wild tomato and up-regulated by salt, drought and osmotic stresses, but repressed by low temperature. Green fluorescent protein (GFP) fusion analysis showed that SpRing is localized at the endoplasmic reticulum. Silencing of SpRing through a virus-induced gene silencing approach led to increased sensitivity to salt stress in wild tomato. Overexpression of SpRing in Arabidopsis thaliana resulted in enhanced salt tolerance during seed germination and early seedling development. The expression levels of certain key stress-related genes are altered both in SpRing-overexpressing Arabidopsis plants and virus-induced gene silenced tomato seedlings. Taken together, our results indicate that SpRing is involved in salt stress and functions as a positive regulator of salt tolerance.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	NA+/H+ ANTIPORTER SOS1 ; TOBACCO-MOSAIC-VIRUS ; ARABIDOPSIS-THALIANA ; CHLOROPHYLL FLUORESCENCE ; HYPERSENSITIVE RESPONSE ; LYCOPERSICON-PENNELLII ; ANTIOXIDATIVE SYSTEM ; TRANSGENIC ALFALFA ; DROUGHT RESPONSE ; UBIQUITIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000374572400008
资助机构	National Project for Transgenic Research [2015ZX08009-003]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25198]
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qi, Shilian,Lin, Qingfang,Zhu, Huishan,et al. The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species[J]. PLANT AND CELL PHYSIOLOGY,2016,57(3):528-539.
APA	Qi, Shilian,Lin, Qingfang,Zhu, Huishan,Gao, Fenghua,Zhang, Wenhao,&Hua, Xuejun.(2016).The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species.PLANT AND CELL PHYSIOLOGY,57(3),528-539.
MLA	Qi, Shilian,et al."The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species".PLANT AND CELL PHYSIOLOGY 57.3(2016):528-539.