Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid | |
Liu, Wenjing2,3; Luo, Haiyun2; Wei, Qinwei1; Liu, Jia2; Wu, Junrong2; Zhang, Yanli2; Chen, Lili4; Ren, Wencai1; Shao, Longquan2,3 | |
刊名 | BIOACTIVE MATERIALS |
2022-03-01 | |
卷号 | 9页码:92-104 |
关键词 | Nanographene oxide Lysophosphatidic acid Hydrogen bonding Endothelial tip cell Hippo signalling |
DOI | 10.1016/j.bioactmat.2021.07.007 |
通讯作者 | Shao, Longquan(shaolongquan@smu.edu.cn) |
英文摘要 | Graphene oxide (GO) exhibits good mechanical and physicochemical characteristics and has extensive application prospects in bone tissue engineering. However, its effect on angiogenesis is unclear, and its potential toxic effects are heavily disputed. Herein, we found that nanographene oxide (NGO) synthesized by one-step water electrolytic oxidation is smaller and shows superior biocompatibility. Moreover, NGO significantly enhanced angiogenesis in calvarial bone defect areas in vivo, providing a good microenvironment for bone regeneration. Endothelial tip cell differentiation is an important step in the initiation of angiogenesis. We verified that NGO activates endothelial tip cells by coupling with lysophosphatidic acid (LPA) in serum via strong hydrogen bonding interactions, which has not been reported. In addition, the mechanism by which NGO promotes angiogenesis was systematically studied. NGO-coupled LPA activates LPAR6 and facilitates the formation of migratory tip cells via Hippo/Yes-associated protein (YAP) independent of reactive oxygen species (ROS) stimulation or additional complex modifications. These results provide an effective strategy for the application of electrochemically derived NGO and more insight into NGO-mediated angiogenesis. |
资助项目 | National Natural Science Foundation of China[52072167] ; National Natural Science Foundation of China[81900989] ; Natural Science Foundation of Guangdong Province[2019A1515011980] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515110088] |
WOS研究方向 | Engineering ; Materials Science |
语种 | 英语 |
出版者 | KEAI PUBLISHING LTD |
WOS记录号 | WOS:000736276600001 |
资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Guangdong Basic and Applied Basic Research Foundation |
内容类型 | 期刊论文 |
源URL | [http://ir.imr.ac.cn/handle/321006/173815] |
专题 | 金属研究所_中国科学院金属研究所 |
通讯作者 | Shao, Longquan |
作者单位 | 1.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Peoples R China 2.Southern Med Univ, Stomatol Hosp, Guangzhou 510280, Peoples R China 3.Guangdong Prov Key Lab Construct & Detect Tissue, Guangzhou 510515, Peoples R China 4.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Stomatol, Wuhan 430022, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Wenjing,Luo, Haiyun,Wei, Qinwei,et al. Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid[J]. BIOACTIVE MATERIALS,2022,9:92-104. |
APA | Liu, Wenjing.,Luo, Haiyun.,Wei, Qinwei.,Liu, Jia.,Wu, Junrong.,...&Shao, Longquan.(2022).Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.BIOACTIVE MATERIALS,9,92-104. |
MLA | Liu, Wenjing,et al."Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid".BIOACTIVE MATERIALS 9(2022):92-104. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论