Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry

doi:10.1021/acs.analchem.6b02502

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry
	Zhou, Ye 6; Wu, Yue 5; Yao, Mingdong 1; Liu, Zheyi 6; Chen, Jin 6; Chen, Jun 1; Tian, Lirong 6; Han, Guangye ; Shen, Jian-Ren ; Wang, Fangjun
刊名	ANALYTICAL CHEMISTRY
	2016
卷号	88 期号:24
ISSN号	0003-2700
DOI	10.1021/acs.analchem.6b02502
文献子类	Article
英文摘要	Positively charged lysines are crucial to maintaining the native structures of proteins and protein complexes by forming hydrogen bonds and electrostatic interactions with their proximal amino acid residues. However, it is still a challenge to develop an efficient method for probing the active proximal microenvironments of lysines without changing their biochemical/physical properties. Herein, we developed an active covalent labeling strategy combined with mass spectrometry to systematically probe the lysine proximal microenvironments within membrane protein complexes (similar to 700 kDa) with high throughput. Our labeling strategy has the advantages of high labeling efficiency and stability, preservation of the active charge states, as well as biological activity of the labeled proteins. In total, 121 lysines with different labeling levels were obtained for the photosystem II complexes from cyanobacteria, red algae, and spinach and provided important insights for understanding the conserved and nonconserved local structures of PSII complexes among evolutionarily divergent species that perform photosynthesis.
学科主题	Chemistry, Analytical
电子版国际标准刊号	1520-6882
出版地	WASHINGTON
WOS关键词	EVOLVING PHOTOSYSTEM-II ; CROSS-LINKED PEPTIDES ; STRUCTURAL-CHARACTERIZATION ; ELECTROSTATIC INTERACTION ; REDUCTIVE METHYLATION ; 33-KDA PROTEIN ; IDENTIFICATION ; CRYSTALLIZATION ; RESIDUES ; DYNAMICS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000390621000015
资助机构	China State Key Basic Research Program Grant [2013CB911203] ; China State Key Research Grant [2016YFA0501402] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21675152, 21305139, 21573223] ; Youth Innovation Promotion Association of CAS [2014164] ; Strategic Priority Research Program of CAS [XDB17030100]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24643]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Energy Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, State Key Lab Catalysis, Dalian 116023, Peoples R China 3.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Okayama Univ, Grad Sch Nat Sci & Technol, Photosynth Res Ctr, 1-1,Naka 3 Chome, Okayama 7008530, Japan 6.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Ye,Wu, Yue,Yao, Mingdong,et al. Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2016,88(24).
APA	Zhou, Ye.,Wu, Yue.,Yao, Mingdong.,Liu, Zheyi.,Chen, Jin.,...&Wang, Fangjun.(2016).Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry.ANALYTICAL CHEMISTRY,88(24).
MLA	Zhou, Ye,et al."Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry".ANALYTICAL CHEMISTRY 88.24(2016).