Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex

doi:10.1104/pp.15.01893

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	Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex
	Shapiguzov, Alexey 3,4,5; Chai, Xin ; Fucile, Geoffrey ; Longoni, Paolo ; Zhang, Lixin ; Rochaix, Jean-David
刊名	PLANT PHYSIOLOGY
	2016
卷号	171 期号:1
ISSN号	0032-0889
DOI	10.1104/pp.15.01893
文献子类	Article
英文摘要	Photosynthetic organisms have the ability to adapt to changes in light quality by readjusting the cross sections of the light-harvesting systems of photosystem II (PSII) and photosystem I (PSI). This process, called state transitions, maintains the redox poise of the photosynthetic electron transfer chain and ensures a high photosynthetic yield when light is limiting. It is mediated by the Stt7/STN7 protein kinase, which is activated through the cytochrome b(6)f complex upon reduction of the plastoquinone pool. Its probable major substrate, the light-harvesting complex of PSII, once phosphorylated, dissociates from PSII and docks to PSI, thereby restoring the balance of absorbed light excitation energy between the two photosystems. Although the kinase is known to be inactivated under high-light intensities, the molecular mechanisms governing its regulation remain unknown. In this study we monitored the redox state of a conserved and essential Cys pair of the Stt7/STN7 kinase and show that it forms a disulfide bridge. We could not detect any change in the redox state of these Cys during state transitions and high-light treatment. It is only after prolonged anaerobiosis that this disulfide bridge is reduced. It is likely to be mainly intramolecular, although kinase activation may involve a transient covalently linked kinase dimer with two intermolecular disulfide bonds. Using the yeast two-hybrid system, we have mapped one interaction site of the kinase on the Rieske protein of the cytochrome b(6)f complex.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	PHOTOSYSTEM-II ; STATE TRANSITIONS ; PROTEIN-PHOSPHORYLATION ; ELECTRON-TRANSFER ; LIGHT ; LHCII ; PHOTOSYNTHESIS ; PLASTOQUINOL ; CHLOROPLASTS ; FLUORESCENCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000375431500006
资助机构	Swiss National FoundationSwiss National Science Foundation (SNSF) [3100AO-117712] ; Foreign Expert Program of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24327]
专题	中科院光生物学重点实验室
作者单位	1.Univ Geneva, Dept Plant Biol, Geneva, Switzerland 2.Univ Helsinki, Dept Biosci, Div Plant Biol, Viikki Plant Sci Ctr, FI-00014 Helsinki, Finland 3.Russian Acad Sci, Inst Plant Physiol, Bot Skaya St 35, Moscow 127276, Russia 4.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing, Peoples R China 5.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland
推荐引用方式 GB/T 7714	Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,et al. Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex[J]. PLANT PHYSIOLOGY,2016,171(1).
APA	Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,Longoni, Paolo,Zhang, Lixin,&Rochaix, Jean-David.(2016).Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex.PLANT PHYSIOLOGY,171(1).
MLA	Shapiguzov, Alexey,et al."Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex".PLANT PHYSIOLOGY 171.1(2016).