Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max

doi:10.1038/srep31134

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	Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max
	Zhang, Yu ; Zhang, Yan-Jie ; Yang, Bao-Jun ; Yu, Xian-Xian ; Wang, Dun 4; Zu, Song-Hao ; Xue, Hong-Wei ; Lin, Wen-Hui
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep31134
文献子类	Article
英文摘要	Brassinosteroids (BRs) play key roles in plant growth and development, and regulate various agricultural traits. Enhanced BR signaling leads to increased seed number and yield in Arabidopsis bzr1-1D (AtBZR1P234L, gain-of-function mutant of the important transcription factor in BR signaling/effects). BR signal transduction pathway is well elucidated in Arabidopsis but less known in other species. Soybean is an important dicot crop producing edible oil and protein. Phylogenetic analysis reveals AtBZR1-like genes are highly conserved in angiosperm and there are 4 orthologues in soybean (GmBZL1-4). We here report the functional characterization of GmBZL2 (relatively highly expresses in flowers). The P234 site in AtBZR1 is conserved in GmBZL2 (P216) and mutation of GmBZL2P216L leads to GmBZL2 accumulation. GmBZL2P216L (GmBZL2) in Arabidopsis results in enhanced BR signaling; including increased seed number per silique. GmBZL2 partially rescued the defects of bri1-5, further demonstrating the conserved function of GmBZL2 with AtBZR1. BR treatment promotes the accumulation, nuclear localization and dephosphorylation/phosphorylation ratio of GmBZL2, revealing that GmBZL2 activity is regulated conservatively by BR signaling. Our studies not only indicate the conserved regulatory mechanism of GmBZL2 and BR signaling pathway in soybean, but also suggest the potential application of GmBZL2 in soybean seed yield.
学科主题	Multidisciplinary Sciences
出版地	BERLIN
WOS关键词	RECEPTOR KINASE ; SEED SIZE ; PLANT-GROWTH ; TRANSDUCTION ; BRASSINOSTEROIDS ; EXPRESSION ; NETWORK ; YIELD ; BZR1 ; ACTIVATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000381006000001
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943404] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371469] ; Opening Research Projects of National Key Laboratory of Plant Molecular Genetics SIPPE, SIBS, CAS ; Shanghai Jiao Tong University ; CSC Scholarship [201404910124]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24347]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA USA 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yu,Zhang, Yan-Jie,Yang, Bao-Jun,et al. Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max[J]. SCIENTIFIC REPORTS,2016,6.
APA	Zhang, Yu.,Zhang, Yan-Jie.,Yang, Bao-Jun.,Yu, Xian-Xian.,Wang, Dun.,...&Lin, Wen-Hui.(2016).Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max.SCIENTIFIC REPORTS,6.
MLA	Zhang, Yu,et al."Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max".SCIENTIFIC REPORTS 6(2016).