GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier

doi:10.1155/2022/2410530

	GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier
	Wang, Xiao-feng 1,2; Hu, Can 1,3; Mo, Shao-Wei 1; Xu, Jing-Li 1; Liu, Yan 1; Xu, Han-dong 1; Yuan, Li 3; Huang, Ling 3; Yu, Jian-fa 3; Cheng, Xiang-Dong 3,4
刊名	JOURNAL OF ONCOLOGY
	2022-01-19
卷号	2022
ISSN号	1687-8450
DOI	10.1155/2022/2410530
通讯作者	Cheng, Xiang-Dong(chengxd@zcmu.edu.cn) ; Xu, Zhi-yuan(xuzy@zjcc.org.cn)
英文摘要	Gastric cancer (GC) is one of the most common types of cancer. The n-butanol extract of Huaier (NEH) is the alcohol-soluble part extracted by the systematic solvent method, which is effective against gastric cancer (GC). However, the mechanism of action of NEH remains unclear. In this study, we aim to evaluate the clinical relevance of GPR30 expression in GC patients and the role of the GPR30/PI3K/AKT signalling pathway in the anti-GC effect of NEH. The expression of GPR30 was examined using immunohistochemistry. Cell counting kit 8 (CCK-8) assay, wound healing, and transwell experiments were used to investigate the viability, migration, and invasion of gastric cancer cells. Western blotting was used to detect the expression of GPR30 and its downstream signalling molecules of the PI3K/AKT signalling pathway. Gastric cancer patient-derived xenografts (PDX) mouse model was used to evaluate the antitumor effect of NEH in vivo. In addition, the graded doses and the maximum tolerated dose of NEH were administered intraperitoneally into the mice for acute toxicity test. We demonstrate that GPR30 expression in GC tissues was significantly higher than that in corresponding adjacent noncancerous tissues and the expression of GPR30 was correlated with a poor prognosis in GC patients. Moreover, GPR30 expression was involved in the migration and invasion of GC cells in vitro. Additionally, we found that NEH can suppress the growth of GC in patient-derived xenograft tumors in vivo. Furthermore, NEH inhibited the proliferation, migration, and invasion in GC cells in a concentration-dependent manner through inhibiting the GPR30-mediated PI3K/AKT signalling pathway in vitro. Acute toxicity test showed that NEH caused no toxic reaction or death and the maximum tolerated dose of NEH in mice was greater than 1600 mg/kg. Our results demonstrate that the high expression of GPR30 is an independent factor of poor prognosis in patients with GC and NEH could be a new agent for the treatment of gastric cancer.
资助项目	Public Platform of Medical Research Center, Zhejiang Chinese Medical University ; Diagnosis and Therapy Center of Upper Gastrointestinal Tumor[JBZX-202006] ; Natural Science Foundation of Zhejiang Province[LY18H290006] ; Key Research and Development Project of Zhejiang Science and Technology Department[2019C03049] ; National Natural Science Foundation of China[81973634] ; National Natural Science Foundation of China[82074245] ; Program of Zhejiang Provincial TCM Sci-Tech Plan[2019ZZ010] ; Program of Zhejiang Provincial TCM Sci-Tech Plan[2021ZA020]
WOS关键词	EXPRESSION ; GROWTH
WOS研究方向	Oncology
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000774898200008
资助机构	Public Platform of Medical Research Center, Zhejiang Chinese Medical University ; Diagnosis and Therapy Center of Upper Gastrointestinal Tumor ; Natural Science Foundation of Zhejiang Province ; Key Research and Development Project of Zhejiang Science and Technology Department ; National Natural Science Foundation of China ; Program of Zhejiang Provincial TCM Sci-Tech Plan
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128695]
专题	中国科学院合肥物质科学研究院
通讯作者	Cheng, Xiang-Dong; Xu, Zhi-yuan
作者单位	1.Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou 310053, Peoples R China 2.Wenzhou Med Univ, Affiliated Cixi Hosp, Cixi Peoples Hosp, Ningbo 315300, Peoples R China 3.Chinese Acad Sci, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC, Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Peoples R China 4.Diag & Therapy Ctr Upper Gastrointestinal Tumor Z, Hangzhou 310022, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiao-feng,Hu, Can,Mo, Shao-Wei,et al. GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier[J]. JOURNAL OF ONCOLOGY,2022,2022.
APA	Wang, Xiao-feng.,Hu, Can.,Mo, Shao-Wei.,Xu, Jing-Li.,Liu, Yan.,...&Xu, Zhi-yuan.(2022).GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier.JOURNAL OF ONCOLOGY,2022.
MLA	Wang, Xiao-feng,et al."GPR30 Activation Promotes the Progression of Gastric Cancer and Plays a Significant Role in the Anti-GC Effect of Huaier".JOURNAL OF ONCOLOGY 2022(2022).