The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem Technology Platform and its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-Blue (TM) Cell Co-Culture Model

doi:10.3389/fphar.2022.820543

	The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem Technology Platform and its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-Blue (TM) Cell Co-Culture Model
	Liu, Ling 1; Lu, Yi 2; Xu, Chao 1; Chen, Haitao 1; Wang, Xuanying 3; Wang, Yijie 3; Cai, Biyu 3; Li, Bing 4; Verstrepen, Lynn 5; Ghyselinck, Jonas 5
刊名	FRONTIERS IN PHARMACOLOGY
	2022-03-15
卷号	13
关键词	chaihu shugan formula gut microbiota gut barrier intestinal immunity SHIMS (R) platform human
DOI	10.3389/fphar.2022.820543
通讯作者	Marzorati, Massimo(Massimo.Marzorati@prodigest.eu) ; Yao, Qinghua(Yaoqh@zjcc.org.cn)
英文摘要	The traditional Chinese medicine (TCM)-Chaihu Shugan Formula (CSF), consisting of several Chinese botanical drugs like Bupleurum, is derived from the ancient Chinese pharmacopeia. It has been used for more than thousands of years in various suboptimal health statuses and diseases induced by chronic stress based on empirical therapy. Recent studies confirm the role of CSF in the development of many diseases, including depression, stress-induced hepatic injury and tumors. However, little has been known about the mechanisms behind the health effects of CSF. Here, we investigate the influence of CSF on the modulation of the simulated colonic microbiota of five healthy donors, gut barrier integrity, and intestinal immunity by combining the simulator of the human intestinal microbial ecosystem (SHIME (R)) technology platform with co-culture of intestinal and immune cells. This approach revealed that CSF stimulated the production of SCFA (acetate, propionate and butyrate) across donors while significantly lowering the production of branched SCFA (bSCFA). In terms of community composition, CSF stimulated a broad spectrum of health-related Bifidobacterium species, which are potent acetate and lactate producers. At the same time, it lowered the abundance of opportunistic pathogenic Escherichia coli. Later, we explore the effect of colonic fermentation of CSF on the gut barrier and intestinal immunity in the Caco-2/THP1-blue (TM) cell co-culture model. Based on the study using SHIME technology platform, CSF showed protective effects on inflammation-induced intestinal epithelial barrier disruption in all donors. Also, the treatment of CSF showed pronounced anti-inflammatory properties by strongly inducing anti-inflammatory cytokines IL-6 and IL-10 and reducing proinflammatory cytokine TNF-alpha. These findings demonstrate a significant modulatory effect of CSF on intestinal gut microbiota. CSF-microbial fermentation products improved the gut barrier and controlled intestinal inflammation.
资助项目	National Natural Science Foundation of China[82074201] ; Chinese Medicine Research Program of Zhejiang Province[2020ZA018]
WOS关键词	TRADITIONAL CHINESE MEDICINE ; SAN ; METABOLISM ; HEALTH
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000783581800001
资助机构	National Natural Science Foundation of China ; Chinese Medicine Research Program of Zhejiang Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128465]
专题	中国科学院合肥物质科学研究院
通讯作者	Marzorati, Massimo; Yao, Qinghua
作者单位	1.Chinese Acad Sci, Dept Integrated Chinese & Western Med, Canc Hosp, Univ Chinese Acad Sci,Zhejiang Canc Hosp,Inst Bas, Hangzhou, Peoples R China 2.Chinese Acad Sci, Dept Clin Nutr, Canc Hosp, Univ Chinese Acad Sci,Zhejiang Canc Hosp,Inst Bas, Hangzhou, Peoples R China 3.Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou, Peoples R China 4.Leuven Hlth Technol Ctr China Ctr, Hangzhou, Peoples R China 5.ProDigest BV, Technol Pk, Zwijnaarde, Belgium 6.Univ Ghent, Fac Biosci Engn, Ctr Microbial Ecol & Technol CMET, Ghent, Belgium 7.Zhejiang Canc Hosp, Key Lab Tradit Chinese Med Oncol, Hangzhou, Peoples R China 8.Key Lab Head & Neck Canc Translat Res Zhejiang Pr, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ling,Lu, Yi,Xu, Chao,et al. The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem Technology Platform and its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-Blue (TM) Cell Co-Culture Model[J]. FRONTIERS IN PHARMACOLOGY,2022,13.
APA	Liu, Ling.,Lu, Yi.,Xu, Chao.,Chen, Haitao.,Wang, Xuanying.,...&Yao, Qinghua.(2022).The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem Technology Platform and its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-Blue (TM) Cell Co-Culture Model.FRONTIERS IN PHARMACOLOGY,13.
MLA	Liu, Ling,et al."The Modulation of Chaihu Shugan Formula on Microbiota Composition in the Simulator of the Human Intestinal Microbial Ecosystem Technology Platform and its Influence on Gut Barrier and Intestinal Immunity in Caco-2/THP1-Blue (TM) Cell Co-Culture Model".FRONTIERS IN PHARMACOLOGY 13(2022).