Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota

doi:10.1111/1462-2920.14962

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	Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota
	Y. Wang,J. B. Xu,L. C. Kong,B. Li,H. Li,W. E. Huang and C. M. Zheng
刊名	Environmental Microbiology
	2020
卷号	22 期号:7 页码:2613-2624
ISSN号	1462-2912
DOI	10.1111/1462-2920.14962
英文摘要	The antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in human gut microbiota have significant impact on human health. While high throughput metagenomic sequencing reveals genotypes of microbial communities, the functionality, phenotype and heterogeneity of human gut microbiota are still elusive. In this study, we applied Raman microscopy and deuterium isotope probing (Raman-DIP) to detect metabolic active ARB (MA-ARB)in situat the single-cell level in human gut microbiota from two healthy adults. We analysed the relative abundances of MA-ARB under different concentrations of amoxicillin, cephalexin, tetracycline, florfenicol and vancomycin. To establish the link between phenotypes and genotypes of the MA-ARB, Raman-activated cell sorting (RACS) was used to sort MA-ARB from human gut microbiota, and mini-metagenomic DNA of the sorted bacteria was amplified, sequenced and analysed. The sorted MA-ARB and their associated ARGs were identified. Our results suggest a strong relation between ARB in human gut microbiota and personal medical history. This study demonstrates that the toolkit of Raman-DIP, RACS and DNA sequencing can be useful to unravel both phenotypes and genotypes of ARB in human gut microbiota at the single-cell level.
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语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/64823]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Y. Wang,J. B. Xu,L. C. Kong,B. Li,H. Li,W. E. Huang and C. M. Zheng. Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota[J]. Environmental Microbiology,2020,22(7):2613-2624.
APA	Y. Wang,J. B. Xu,L. C. Kong,B. Li,H. Li,W. E. Huang and C. M. Zheng.(2020).Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota.Environmental Microbiology,22(7),2613-2624.
MLA	Y. Wang,J. B. Xu,L. C. Kong,B. Li,H. Li,W. E. Huang and C. M. Zheng."Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota".Environmental Microbiology 22.7(2020):2613-2624.