AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells | |
Li M(李密); Xiao XB(肖秀斌); Zhang WJ(张伟京); Liu LQ(刘连庆); Wang YC(王越超) | |
刊名 | CELLULAR IMMUNOLOGY |
2014 | |
卷号 | 290期号:2页码:233-244 |
关键词 | Atomic Force Microscopy Antibody-dependent Cellular Cytotoxicity Lymphoma Natural Killer Cell Cd20 Fc Receptor |
ISSN号 | 0008-8749 |
产权排序 | 1 |
英文摘要 | Rituximab is a monoclonal antibody drug approved for the treatment of patients with lymphomas. Rituximab's main killing mechanism is antibody-dependent cellular cytotoxicity (ADCC). During ADCC, rituximab's fragment antigen binding (Fab) region binds to the CD20 antigen on the tumor cell and its fragment crystallizable (Fc) region binds to the Fc receptor (FcR) on the natural killer (NK) cells. In this study, two types of molecular interactions (CD20-rituximab, FcR-rituximab) involved in ADCC were measured simultaneously on cells prepared from biopsy specimens of lymphoma patients by utilizing atomic force microscopy (AFM) with functionalized tips carrying rituximab. NK cells were detected by specific NKp46 fluorescent labeling and tumor cells were detected by specific ROR1 fluorescent labeling. Based on the fluorescence recognition, the binding affinity and distribution of FcRs on NK cells, and CD20 on tumor cells, were quantitatively measured and mapped. The binding affinity and distribution of FcRs (on NK cells) and CD20 (on tumor cells) were associated with rituximab clinical efficacy. The experimental results provide a new approach to simultaneously quantify the multiple types of molecular interactions involved in rituximab ADCC mechanism on patient biopsy cells, which is of potential clinical significance to predict rituximab efficacy for personalized medicine. (C) 2014 Published by Elsevier Inc. |
WOS关键词 | ATOMIC-FORCE MICROSCOPY ; NATURAL-KILLER-CELLS ; FC-GAMMA RECEPTORS ; ANTI-CD20 MONOCLONAL-ANTIBODIES ; SINGLE-MOLECULE ; LIVING CELLS ; CANCER-CELLS ; SURFACE EXPRESSION ; RECOGNITION EVENTS ; CLINICAL-EFFICACY |
WOS研究方向 | Cell Biology ; Immunology |
语种 | 英语 |
WOS记录号 | WOS:000342955000009 |
资助机构 | National Natural Science Foundation of China [61175103, 61327014] ; CAS FEA International Partnership Program for Creative Research Teams |
公开日期 | 2014-11-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/15268] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
作者单位 | 1.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China 2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China 3.University of Chinese Academy of Sciences, Beijing 100049, China 4.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China |
推荐引用方式 GB/T 7714 | Li M,Xiao XB,Zhang WJ,et al. AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells[J]. CELLULAR IMMUNOLOGY,2014,290(2):233-244. |
APA | Li M,Xiao XB,Zhang WJ,Liu LQ,&Wang YC.(2014).AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells.CELLULAR IMMUNOLOGY,290(2),233-244. |
MLA | Li M,et al."AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells".CELLULAR IMMUNOLOGY 290.2(2014):233-244. |
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