Isolation, Identification and Characterization of Two Kinds of Deep-Sea Bacterial Lipopeptides Against Foodborne Pathogens

doi:10.3389/fmicb.2022.792755

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Isolation, Identification and Characterization of Two Kinds of Deep-Sea Bacterial Lipopeptides Against Foodborne Pathogens
	Gu, Yanjun 4; Zheng, Rikuan 1,2,3; Sun, Chaomin 1,2,3; Wu, Shimei 4
刊名	FRONTIERS IN MICROBIOLOGY
	2022-02-03
卷号	13 页码:11
关键词	Bacillus deep-sea lipopeptides foodborne pathogens fengycin surfactin
DOI	10.3389/fmicb.2022.792755
通讯作者	Wu, Shimei(shimeiwu2016@126.com)
英文摘要	Under multiple stresses of deep sea, many microorganisms have evolved potentials to produce different metabolites to cope with the stresses they face. In this study, we isolated a bacterial strain Bacillus sp. YJ17 from the deep-sea cold seep. Compared with commercial food preservative nisin, it showed broad and strong antibacterial activities against foodborne pathogens, including multiple resistant bacteria Pseudomonas aeruginosa PAO1 and methicillin-resistant Staphylococcus aureus (MRSA). The active agents were purified by reversed-phase high performance liquid chromatography (RP-HPLC). Analysis of high-energy collision induced dissociation mass spectrometry (HCD-MS) showed that the two active agents belong to family of fengycin and surfactin, and based on results of tandem mass spectrometry (HCD-MS/MS), the amino acid sequence of purified fengycin and surfactin might be Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Tyr-Ile and Glu-Leu/Ile-Leu/Ile-Leu/Ile-Val-Asp-Leu/Ile, respectively. Since the purified fengycin and surfactin exhibited strong inhibition against P. aeruginosa PAO1 and MRSA respectively, the inhibition mechanisms of fengycin against P. aeruginosa PAO1 and surfactin against MRSA were investigated by electron microscopy. After treatment with purified fengycin, the morphology of P. aeruginosa PAO1 became abnormal and aggregated together, and obvious cytoplasmic leakage was observed. After treatment with purified surfactin, the MRSA cells clustered together, and cell surface became rough and jagged. Further study showed that reactive oxygen species (ROS) accumulation and cell membrane damage occurred in P. aeruginosa PAO1 and MRSA after treated with fengycin and surfactin, respectively. Furthermore, typical ROS scavenging enzymes catalase (CAT) and superoxide dismutase (SOD) were also significantly reduced in P. aeruginosa PAO1 and MRSA after treated with fengycin and surfactin, respectively. Therefore, the inhibition mechanisms of fengycin against P. aeruginosa PAO1 and surfactin against MRSA are closely related with accumulation of ROS, which might be due to the decreased activity of CAT and SOD after treated with fengycin and surfactin, respectively. Overall, our study provides good candidates from the deep-sea environment to deal with foodborne pathogens, especially multidrug-resistant bacteria.
资助项目	Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; National Key R&D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; National Natural Science Foundation of China[31600035]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000759924800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178026]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wu, Shimei
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 4.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Gu, Yanjun,Zheng, Rikuan,Sun, Chaomin,et al. Isolation, Identification and Characterization of Two Kinds of Deep-Sea Bacterial Lipopeptides Against Foodborne Pathogens[J]. FRONTIERS IN MICROBIOLOGY,2022,13:11.
APA	Gu, Yanjun,Zheng, Rikuan,Sun, Chaomin,&Wu, Shimei.(2022).Isolation, Identification and Characterization of Two Kinds of Deep-Sea Bacterial Lipopeptides Against Foodborne Pathogens.FRONTIERS IN MICROBIOLOGY,13,11.
MLA	Gu, Yanjun,et al."Isolation, Identification and Characterization of Two Kinds of Deep-Sea Bacterial Lipopeptides Against Foodborne Pathogens".FRONTIERS IN MICROBIOLOGY 13(2022):11.