Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana

doi:10.3390/ijms23020654

	Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana
	Du, Yan 1; Feng, Zhuo 1,2; Wang, Jie 1; Jin, Wenjie 1; Wang, Zhuanzi 1; Guo, Tao 3; Chen, Yuze 1; Feng, Hui 1,2; Yu, Lixia 1; Li, Wenjian 1
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2022
卷号	23 期号:2 页码:22
关键词	gamma rays mutation phenotype screening whole-genome re-sequencing Arabidopsis thaliana
DOI	10.3390/ijms23020654
通讯作者	Zhou, Libin(libinzhoulz@gmail.com)
英文摘要	Genetic variations are an important source of germplasm diversity, as it provides an allele resource that contributes to the development of new traits for plant breeding. Gamma rays have been widely used as a physical agent for mutation creation in plants, and their mutagenic effect has attracted extensive attention. However, few studies are available on the comprehensive mutation profile at both the large-scale phenotype mutation screening and whole-genome mutation scanning. In this study, biological effects on M-1 generation, large-scale phenotype screening in M-2 generation, as well as whole-genome re-sequencing of seven M-3 phenotype-visible lines were carried out to comprehensively evaluate the mutagenic effects of gamma rays on Arabidopsis thaliana. A total of 417 plants with visible mutated phenotypes were isolated from 20,502 M-2 plants, and the phenotypic mutation frequency of gamma rays was 2.03% in Arabidopsis thaliana. On average, there were 21.57 single-base substitutions (SBSs) and 11.57 small insertions and deletions (InDels) in each line. Single-base InDels accounts for 66.7% of the small InDels. The genomic mutation frequency was 2.78 x 10(-10)/bp/Gy. The ratio of transition/transversion was 1.60, and 64.28% of the C > T events exhibited the pyrimidine dinucleotide sequence; 69.14% of the small InDels were located in the sequence with 1 to 4 bp terminal microhomology that was used for DNA end rejoining, while SBSs were less dependent on terminal microhomology. Nine genes, on average, were predicted to suffer from functional alteration in each re-sequenced line. This indicated that a suitable mutation gene density was an advantage of gamma rays when trying to improve elite materials for one certain or a few traits. These results will aid the full understanding of the mutagenic effects and mechanisms of gamma rays and provide a basis for suitable mutagen selection and parameter design, which can further facilitate the development of more controlled mutagenesis methods for plant mutation breeding.
WOS关键词	FUNCTIONAL GENOMICS ; IONIZING-RADIATION ; BEAM IRRADIATION ; ION IRRADIATION ; MECHANISMS ; EFFICIENCY ; PLANTS ; REPAIR ; GENE ; END
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000747260400001
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/142290]
专题	中国科学院近代物理研究所
通讯作者	Zhou, Libin
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Biomed Ctr, Biophys Grp, Lanzhou 730000, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100045, Peoples R China 3.South China Agr Univ, Natl Engn Res Ctr Plant Space Breeding, Guangzhou 510640, Peoples R China 4.Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China
推荐引用方式 GB/T 7714	Du, Yan,Feng, Zhuo,Wang, Jie,et al. Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(2):22.
APA	Du, Yan.,Feng, Zhuo.,Wang, Jie.,Jin, Wenjie.,Wang, Zhuanzi.,...&Zhou, Libin.(2022).Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(2),22.
MLA	Du, Yan,et al."Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.2(2022):22.