In view of the background interference of the current multi-point laser target source positioning detection system, positioning accuracy and real-time performance need to be improved. Based on the DM6437 hardware platform, an embedded multi-point laser target positioning system is designed. The collection of image information is completed by CMOS camera and TVP5158 chip. The DM6437 chip realizes tasks such as algorithm processing and data transmission. Experimental results show that the target location algorithm based on threshold and shape effectively removes the interfering light source in the image background. Therefore, the accuracy of laser target location is greatly improved. The positioning system based on the DSP hardware platform is small in size, stable in operation, and has high application promotion value. © 2022 SPIE