Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation

doi:10.1002/elsc.202000104

	Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation
	Zhang, Bingyang 3; Yin, Shuang 3; Wang, Yingli 2; Su, Zhiguo 1; Bi, Jingxiu 3
刊名	ENGINEERING IN LIFE SCIENCES
	2021-05-03
页码	15
关键词	hepatitis B core molecular dynamic simulation protein characterization protein purification virus‐ like particle
ISSN号	1618-0240
DOI	10.1002/elsc.202000104
英文摘要	Inserting foreign epitopes to hepatitis B core (HBc) virus-like particles (VLPs) could influence the molecular conformation and therefore vary the purification process. In this study, a cost-effective purification process was developed for two chimeric HBc VLPs displaying Epstein-Barr nuclear antigens 1 (EBNA1), and hepatitis C virus (HCV) core. Both chimeric VLPs were expressed in soluble form with high production yields in Escherichia coli. Molecular dynamic (MD) simulation was employed to predict the stability of chimeric VLPs. HCV core-HBc was found to be less stable in water environment compared with EBNA1-HBc, indicating its higher hydrophobicity. Assisting with MD simulation, ammonium sulfate precipitation was optimized to remove host cell proteins with high target protein recovery yields. Moreover, 99% DNA impurities were removed using POROS 50 HQ chromatography. In characterization measurement, we found that inserting HCV core epitope would reduce the ratio of alpha-helix of HCV core-HBc. This could be another reason on the top of its higher hydrophobicity predicted by MD simulation, causing its less stability. Tertiary structure, transmission electron microscopy, and immunogenicity results indicate that two chimeric VLPs maintained correct VLP structure ensuring its bioactivity after being processed by the developed cost-effective purification approach.
资助项目	University of Adelaide ; National Natural Science Foundation of China[21576267] ; Institute of Process Engineering, Chinese Academy of Sciences ; Shanxi Education Science "1331 project" special research project (Research and Development of Traditional Chinese Medicine Micro-emulsion and New Biological Preparation)
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000646247700001
资助机构	University of Adelaide ; National Natural Science Foundation of China ; Institute of Process Engineering, Chinese Academy of Sciences ; Shanxi Education Science "1331 project" special research project (Research and Development of Traditional Chinese Medicine Micro-emulsion and New Biological Preparation)
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/48484]
专题	中国科学院过程工程研究所
通讯作者	Bi, Jingxiu
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 2.Shanxi Univ Tradit Chinese Med, Sch Chinese Med & Food Engn, Jinzhong, Shanxi, Peoples R China 3.Univ Adelaide, Fac Engn Comp & Math Sci, Sch Chem Engn & Adv Mat, Adelaide, SA 5005, Australia
推荐引用方式 GB/T 7714	Zhang, Bingyang,Yin, Shuang,Wang, Yingli,et al. Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation[J]. ENGINEERING IN LIFE SCIENCES,2021:15.
APA	Zhang, Bingyang,Yin, Shuang,Wang, Yingli,Su, Zhiguo,&Bi, Jingxiu.(2021).Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation.ENGINEERING IN LIFE SCIENCES,15.
MLA	Zhang, Bingyang,et al."Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation".ENGINEERING IN LIFE SCIENCES (2021):15.