Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology

doi:10.1021/acssynbio.0c00158

	Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology
	Wu, Fengli 1,2; Chen, Wujiu 1; Peng, Yanfeng 1; Tu, Ran 1; Lin, Yuping 1; Xing, Jianmin 2; Wang, Qinhong 1
刊名	ACS SYNTHETIC BIOLOGY
	2020-09-18
卷号	9 期号:9 页码:2399-2409
关键词	Vibrio natriegens synthetic regulatory parts combinatorial modulation glucose transport synthetic biology
ISSN号	2161-5063
DOI	10.1021/acssynbio.0c00158
英文摘要	The fast-growing Vibrio natriegens is an attractive robust chassis for diverse synthetic biology applications. However, V. natriegens lacks the suitable constitutive regulatory parts for precisely tuning the gene expression and, thus, recapitulating physiologically relevant changes in gene expression levels. In this study, we designed, constructed, and screened the synthetic regulatory parts by varying the promoter region and ribosome binding site element for V. natriegens with different transcriptional or translational strengths, respectively. The fluorescence intensities of the cells with different synthetic regulatory parts could distribute evenly over a wide range of 5 orders of magnitude. The selected synthetic regulatory parts had good stability in both nutrient-rich and minimal media. The precise combinatorial modulation of galP (GalP = galactose permease) and glk (Glk = glucokinase) from Escherichia coli by using three synthetic regulatory parts with different strengths was confirmed in a phosphoenolpyruvate:carbohydrate phosphotransferase system with inactive V. natriegens strain to alter the glucose transport. This work provides the simple, efficient, and standardized constitutive regulatory parts for V. natriegens synthetic biology.
资助项目	National Natural Science Foundation of China[31700080] ; China Postdoctoral Science Foundation[2017M621082] ; Enterprise Postdoctoral Innovation Project of Tianjin[TJQYBSH2017006]
WOS关键词	EXPRESSION ; SEQUENCE ; PROTEIN ; TRANSLATION ; BACTERIUM ; PROMOTERS ; GLUCOSE
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000574922400019
资助机构	National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Enterprise Postdoctoral Innovation Project of Tianjin
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42245]
专题	中国科学院过程工程研究所
通讯作者	Wang, Qinhong
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, CAS Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wu, Fengli,Chen, Wujiu,Peng, Yanfeng,et al. Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology[J]. ACS SYNTHETIC BIOLOGY,2020,9(9):2399-2409.
APA	Wu, Fengli.,Chen, Wujiu.,Peng, Yanfeng.,Tu, Ran.,Lin, Yuping.,...&Wang, Qinhong.(2020).Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology.ACS SYNTHETIC BIOLOGY,9(9),2399-2409.
MLA	Wu, Fengli,et al."Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology".ACS SYNTHETIC BIOLOGY 9.9(2020):2399-2409.