From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability

doi:10.1016/j.memsci.2020.118287

	From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability
	Wang, Jingyu 1,2; Luo, Jianquan 1,2; Ren, Yuling 1; Su, Ziran 3; Guo, Shiwei 1; Woodley, John M.3; Wan, Yinhua 1
刊名	JOURNAL OF MEMBRANE SCIENCE
	2020-09-01
卷号	610 页码:10
关键词	Ultrafiltration Antifouling Surface modification Polydopamine Enzyme reuse
ISSN号	0376-7388
DOI	10.1016/j.memsci.2020.118287
英文摘要	A by-product from membrane-mediated molasses refining process mainly contains sucrose, glucose, fructose and KCl, which is an ideal raw material to produce high fructose syrup (HFS) by invertase. Ultrafiltration (UF) membrane can achieve invertase reuse while protein adsorption on the membrane not only decreases the membrane permeability, but also induces enzyme activity loss. In this work, the effect of glucose/fructose and KCl on the catalytic behavior of invertase was investigated. Then the reusability of invertase for sucrose hy-drolysis was evaluated by different UF membranes, and mussel-inspired surface modification was employed to further improve the invertase reusability. The results showed that the presence of KCl in the raw materials greatly decreased the reaction rate, and thus desalination and concentration of the raw materials should be conducted before enzymatic hydrolysis to improve the enzymatic hydrolysis efficiency, save energy and reduce reactor volume. Moreover, it was found that the sucrose conversion decline with enzyme reuse by UF was caused by enzyme adsorption in/on the membrane and/or its penetration through the membrane leading to overall enzyme loss in the retentate. A polyether sulfone (PES) membrane with molecular weight cut-off (MWCO) of 10 kDa (UP010) was selected to recycle invertase because it enabled complete invertase retention and relatively high permeate flux. When L-cysteine (Cys) was grafted on the polydopamine-coated UP010 membrane, it could reduce invertase adsorption on the membrane, and thus keep high sucrose conversion (93-95%) in four enzyme reuse cycles because the grafted Cys greatly increased the negative charge and hydrophilicity of the membrane surface. This work not only offered an alternative for HFS production by membrane technology but also developed a novel strategy to construct antifouling surface to retard enzyme adsorption.
资助项目	Guangxi Science and Technology Major Project[AA17204034]
WOS关键词	ENZYME IMMOBILIZATION ; ANTIFOULING PROPERTY ; MOLECULAR-WEIGHT ; CANE MOLASSES ; FILTRATION ; REMOVAL ; HYDROLYSIS ; DOPAMINE ; COATINGS ; SUCROSE
WOS研究方向	Engineering ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000555548500006
资助机构	Guangxi Science and Technology Major Project
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41630]
专题	中国科学院过程工程研究所
通讯作者	Luo, Jianquan; Wan, Yinhua
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China 3.Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark
推荐引用方式 GB/T 7714	Wang, Jingyu,Luo, Jianquan,Ren, Yuling,et al. From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability[J]. JOURNAL OF MEMBRANE SCIENCE,2020,610:10.
APA	Wang, Jingyu.,Luo, Jianquan.,Ren, Yuling.,Su, Ziran.,Guo, Shiwei.,...&Wan, Yinhua.(2020).From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability.JOURNAL OF MEMBRANE SCIENCE,610,10.
MLA	Wang, Jingyu,et al."From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability".JOURNAL OF MEMBRANE SCIENCE 610(2020):10.