Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells

doi:10.1093/rb/rbz026

	Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells
	Lei, Xiongxin 1; Gao, Jianping 1,2; Xing, Fangyu 3; Zhang, Yang 4; Ma, Ye 5; Zhang, Guifeng 1,2
刊名	REGENERATIVE BIOMATERIALS
	2019-12-01
卷号	6 期号:6 页码:361-371
关键词	nano-hydroxyapatite natural bone ceramic collagen scaffold MC3T3-E1 osteoblasts
ISSN号	2056-3418
DOI	10.1093/rb/rbz026
英文摘要	The use of various types of calcium phosphate has been reported in the preparation of repairing materials for bone defects. However, the physicochemical and biological properties among them might be vastly different. In this study, we prepared two types of calcium phosphates, nano-hydroxyapatite (nHA) and natural bone ceramic (NBC), into 3D scaffolds by mixing with type I collagen (CoL), resulting in the nHA/CoL and NBC/CoL scaffolds. We then evaluated and compared the physicochemical and biological properties of these two calcium phosphates and their composite scaffold with CoL. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD) and compressive tests were used to, respectively, characterize the morphology, composition, distribution and the effect of nHA and NBC to collagen. Next, we examined the biological properties of the scaffolds using cytotoxicity testing, flow cytometry, immunofluorescence staining, biocompatibility testing, CCK-8 assays and RT-PCR. The results reflected that the Ca2+ released from nHA and NBC could bind chemically with collagen and affect its physicochemical properties, including the infrared absorption spectrum and compression modulus, among others. Furthermore, the two kinds of scaffolds could promote the expression of osteo-relative genes, but showed different gene induction properties. In short, NBC/CoL could promote the expression of early osteogenic genes, while nHA/CoL could upregulate late osteogenic genes. Conclusively, these two composite scaffolds could provide MC3T3-E1 cells with a biomimetic surface for adhesion, proliferation and the formation of mineralized extracellular matrices. Moreover, nHA/CoL and NBC/CoL had different effects on the period and extent ofMC3T3-E1 cell mineralization.
资助项目	National Key Research and Development Program of China[2018YFA0108200] ; National Key Research and Development Program of China[2018YFC116400] ; International Cooperation Project of Hainan Province[KJHZ2015-30] ; Sci&Tech Program of Guangzhou of China[201803010086]
WOS关键词	SELF-ORGANIZATION MECHANISM ; CALCIUM-PHOSPHATE CEMENTS ; IN-VITRO ; DRUG-DELIVERY ; COLLAGEN ; SPECTROSCOPY ; COMPOSITES ; PARTICLES ; CERAMICS ; MATRICES
WOS研究方向	Materials Science
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000517151900005
资助机构	National Key Research and Development Program of China ; International Cooperation Project of Hainan Province ; Sci&Tech Program of Guangzhou of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/39434]
专题	中国科学院过程工程研究所
通讯作者	Zhang, Guifeng
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing 100049, Peoples R China 3.Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China 4.Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China 5.Guandong Pharmaceut Univ, Sch Basic Course, Dept Pathogen Biol & Immunol, Guangzhou 510006, Peoples R China
推荐引用方式 GB/T 7714	Lei, Xiongxin,Gao, Jianping,Xing, Fangyu,et al. Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells[J]. REGENERATIVE BIOMATERIALS,2019,6(6):361-371.
APA	Lei, Xiongxin,Gao, Jianping,Xing, Fangyu,Zhang, Yang,Ma, Ye,&Zhang, Guifeng.(2019).Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells.REGENERATIVE BIOMATERIALS,6(6),361-371.
MLA	Lei, Xiongxin,et al."Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells".REGENERATIVE BIOMATERIALS 6.6(2019):361-371.