The Novel Chinese Medicine JY5 Formula Alleviates Hepatic Fibrosis by Inhibiting the Notch Signaling Pathway

doi:10.3389/fphar.2021.671152

	The Novel Chinese Medicine JY5 Formula Alleviates Hepatic Fibrosis by Inhibiting the Notch Signaling Pathway
	Fu, Yadong 3,4,5; Xiao, Zhun 3,4,5; Tian, Xiaoting 2; Liu, Wei 3,5; Xu, Zhou 2; Yang, Tao 1; Hu, Yonghong 3,5; Zhou, Xiaoxi 3,5; Fang, Jing 3,5; Gao, Siqi 3,4,5
刊名	FRONTIERS IN PHARMACOLOGY
	2021-09-22
卷号	12 页码:15
关键词	traditional Chinese medicine JY5 formula fuzheng huayu hepatic fibrosis notch signaling pathway
DOI	10.3389/fphar.2021.671152
通讯作者	Huang, Chenggang(cghuang3208@126.com) ; Chen, Jiamei(cjm0102@126.com) ; Liu, Ping(liuliver@vip.sina.com)
英文摘要	Advanced liver fibrosis can lead to cirrhosis, resulting in an accelerated risk of hepatocellular carcinoma and liver failure. Fuzheng Huayu formula (FZHY) is a traditional Chinese medicine formula treated liver fibrosis in China approved by a Chinese State Food and Drug Administration (NO: Z20050546), composed of Salvia Miltiorrhiza bge., Prunus davidiana (Carr.) Franch., cultured Cordyceps sinensis (BerK.) Sacc. Mycelia, Schisandra chinensis (Turcz.) Baill., Pinus massoniana Lamb., and Gynostemma pentaphyllum (Thunb.) Makino. However, the main active substances and mechanism of FZHY are unclear. The aim of this study is to identify a novel anti-fibrotic compound, which consists of the main active ingredients of FZHY, and investigate its mechanism of pharmacological action. The main active ingredients of FZHY were investigated by quantitative analysis of FZHY extracts and FZHY-treated plasma and liver in rats. The anti-fibrotic composition of the main active ingredients was studied through uniform design in vivo, and its mechanism was evaluated in carbon tetrachloride (CCl4)- and bile duct ligation (BDL)-induced liver fibrosis models in rats and mice, and transforming growth factor beta 1-induced LX-2 cell activation model in vitro. A novel Chinese medicine, namely JY5 formula, consisting of salvianolic acid B, schisantherin A, and amygdalin, the main active ingredients of FZHY, significantly alleviated hepatic hydroxyproline content and collagen deposition in CCl4-and BDL-induced fibrotic liver in rats and mice. In addition, JY5 inhibited the activation of hepatic stellate cells (HSCs) by inactivating Notch signaling in vitro and in vivo. In this study, we found a novel JY5 formula, which exerted anti-hepatic fibrotic effects by inhibiting the Notch signaling pathway, consequently suppressing HSCs activation. These results provide an adequate scientific basis for clinical research and application of the JY5 formula, which may be a potential novel therapeutic candidate for liver fibrosis.
资助项目	National Natural Science Foundation of China[81530101] ; National Natural Science Foundation of China[81973613] ; Shanghai Rising-Star Program[19QA1408900] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2019ZX09201001-001-008] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2019280]
WOS关键词	SALVIANOLIC ACID B ; LIVER FIBROSIS ; TRANSFORMATION ; CELLS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000721536900001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/299006]
专题	中国科学院上海药物研究所
通讯作者	Huang, Chenggang; Chen, Jiamei; Liu, Ping
作者单位	1.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Dept Cardiol, Cardiovasc Res Inst, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Inst Liver Dis, Minist Educ,Key Lab Liver & Kidney Dis, Shanghai, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, Shanghai, Peoples R China 5.Shanghai Key Lab Tradit Chinese Clin Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Fu, Yadong,Xiao, Zhun,Tian, Xiaoting,et al. The Novel Chinese Medicine JY5 Formula Alleviates Hepatic Fibrosis by Inhibiting the Notch Signaling Pathway[J]. FRONTIERS IN PHARMACOLOGY,2021,12:15.
APA	Fu, Yadong.,Xiao, Zhun.,Tian, Xiaoting.,Liu, Wei.,Xu, Zhou.,...&Liu, Ping.(2021).The Novel Chinese Medicine JY5 Formula Alleviates Hepatic Fibrosis by Inhibiting the Notch Signaling Pathway.FRONTIERS IN PHARMACOLOGY,12,15.
MLA	Fu, Yadong,et al."The Novel Chinese Medicine JY5 Formula Alleviates Hepatic Fibrosis by Inhibiting the Notch Signaling Pathway".FRONTIERS IN PHARMACOLOGY 12(2021):15.