Progression and Regression of Abdominal Aortic Aneurysms in Mice

doi:10.1007/s11596-021-2425-z

	Progression and Regression of Abdominal Aortic Aneurysms in Mice
	Ding, Yu-chao 1,2; Zhang, Xian-jing 1; Zhang, Ji-xiu 1; Zhai, Zi-yi 1,2; Zhang, Mei-xia 2; Jiang, Bao-hong 1
刊名	CURRENT MEDICAL SCIENCE
	2021-10-13
页码	8
关键词	abdominal aortic aneurysm disease progression disease regression elastogenesis lysyl oxidase
ISSN号	2096-5230
DOI	10.1007/s11596-021-2425-z
通讯作者	Zhang, Mei-xia(mxzhang@cmu.edu.cn) ; Jiang, Bao-hong(jiangbh@simm.ac.cn)
英文摘要	Objective Abdominal aortic aneurysm (AAA) is a significant medical problem with a high mortality rate. Nevertheless, the underlying mechanism for the progression and regression of AAA is unknown. Methods Experimental model of AAA was first created by porcine pancreatic elastase incubation around the infrarenal aorta of C57BL/6 mice. Then, AAA progression and regression were evaluated based on the diameter and volume of AAA. The aortas were harvested for hematoxylin-eosin staining (HE), orcein staining, sirius red staining, immunofluorescence analysis and perls' prussian blue staining at the indicated time point. Finally, beta-aminopropionitrile monofumarate (BAPN) was used to explore the underlying mechanism of the regression of AAA. Results When we extended the observation period to 100 days, we not only observed an increase in the AAA diameter and volume in the early stage, but also a decrease in the late stage. Consistent with AAA diameter and volume, the aortic thickness showed the same tendency based on HE staining. The elastin and collagen content first degraded and then regenerated, which corresponds to the early deterioration and late regression of AAA. Then, endogenous up-regulation of lysyl oxidase (LOX) was detected, accompanying the regression of AAA, as detected by an immunofluorescent assay. BAPN and LOX inhibitor considerably inhibited the regression of AAA, paralleling the degradation of elastin lamella and collagen. Conclusion Taken together, we tentatively conclude that endogenous re-generation of LOX played an influential role in the regression of AAA. Therefore, regulatory factors on the generation of LOX exhibit promising therapeutic potential against AAA.
资助项目	National Natural Science Foundation of China[81770478] ; National Natural Science Foundation of China[81970395] ; Shanghai Science and Technology Development Foundation[21S21901900] ; Inter-governmental Science and Technology Innovation Cooperation Program, Ministry of Science and Technology of China[2021YFE0111300] ; Biological Resources Programme, Chinese Academy of Sciences[KFJ-BRP-008]
WOS关键词	LYSYL OXIDASE ; MATRIX ; MODEL ; INHIBITION ; CELLS
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000706939900001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298427]
专题	中国科学院上海药物研究所
通讯作者	Zhang, Mei-xia; Jiang, Bao-hong
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.China Med Univ, Coll Basic Med Sci, Shenyang 110122, Peoples R China
推荐引用方式 GB/T 7714	Ding, Yu-chao,Zhang, Xian-jing,Zhang, Ji-xiu,et al. Progression and Regression of Abdominal Aortic Aneurysms in Mice[J]. CURRENT MEDICAL SCIENCE,2021:8.
APA	Ding, Yu-chao,Zhang, Xian-jing,Zhang, Ji-xiu,Zhai, Zi-yi,Zhang, Mei-xia,&Jiang, Bao-hong.(2021).Progression and Regression of Abdominal Aortic Aneurysms in Mice.CURRENT MEDICAL SCIENCE,8.
MLA	Ding, Yu-chao,et al."Progression and Regression of Abdominal Aortic Aneurysms in Mice".CURRENT MEDICAL SCIENCE (2021):8.