Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

doi:10.3390/v14010108

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments
	Liu, Wang 2,3; Zheng, Xiaowei 2; Dai, Xin 2,3; Zhang, Zhenfeng 2; Zhang, Wenyan 1,4,5; Xiao, Tian 1,4,5; Huang, Li 2,3
刊名	VIRUSES-BASEL
	2022
卷号	14 期号:1 页码:14
关键词	Psychrobacillus bacteriophage marine sediments Myoviridae
DOI	10.3390/v14010108
通讯作者	Huang, Li(huangl@im.ac.cn)
英文摘要	Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3 '-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (>= 70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.
资助项目	National Key R&D Program of China[2018YFC0310703] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; China Ocean Mineral Resources RD Association[DY135-B2-02] ; National Natural Science Foundation of China[31970170] ; National Natural Science Foundation of China[92051109] ; National Natural Science Foundation of China[92151001] ; National Natural Science Foundation of China[KEX-UE2019GZ05] ; National Natural Science Foundation of China[KEXUE2019GZ03]
WOS研究方向	Virology
语种	英语
出版者	MDPI
WOS记录号	WOS:000747457100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177711]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Huang, Li
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266273, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wang,Zheng, Xiaowei,Dai, Xin,et al. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments[J]. VIRUSES-BASEL,2022,14(1):14.
APA	Liu, Wang.,Zheng, Xiaowei.,Dai, Xin.,Zhang, Zhenfeng.,Zhang, Wenyan.,...&Huang, Li.(2022).Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments.VIRUSES-BASEL,14(1),14.
MLA	Liu, Wang,et al."Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments".VIRUSES-BASEL 14.1(2022):14.