microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation

doi:10.1016/j.fsi.2021.10.025

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation
	Chen, Hao 1,3,4,5; Wang, Minxiao 1,3,4,5; Zhang, Huan 1,3,4,5; Wang, Hao 1,3,4,5; Zhou, Li 1,3,4,5; Zhong, Zhaoshan 1,2,3,4,5; Cao, Lei 1,3,4,5; Lian, Chao 1,3,4,5; Sun, Yan 1,3,4,5; Li, Chaolun 1,2,3,4,5
刊名	FISH & SHELLFISH IMMUNOLOGY
	2021-12-01
卷号	119 页码:420-431
关键词	Bathymodiolinae mussel Pattern recognition receptor Methane oxidation bacteria Chemosynthetic ecosystem miRNAs Symbiosis
ISSN号	1050-4648
DOI	10.1016/j.fsi.2021.10.025
通讯作者	Li, Chaolun(lcl@qdio.ac.cn)
英文摘要	Bathymodiolinae mussels are dominant species in cold seeps and hydrothermal vents and could harbor endosymbionts in gill bacteriocytes. However, mechanisms underlying the symbiosis have remained largely undisclosed for years. In the present study, the global expression pattern of immune-related genes and miRNAs were surveyed in Gigantidas platifrons during bacterial challenges using enriched symbiotic methane oxidation bacteria MOBs or nonsymbiotic Vibrio. As a result, multiple pattern recognition receptors were found differentially expressed at 12 h and 24 h post bacteria challenges and distinctly clustered between stimulations. Dozens of immune effectors along with signal transducers were also modulated simultaneously during MOB or Vibrio challenge. A total of 459 miRNAs were identified in the gill while some were differentially expressed post MOB or nonsymbiotic bacteria challenge. A variety of immune-related genes were annotated as target genes of aforesaid differentially expressed miRNAs. As a result, biological processes including the immune recognition, lysosome activity and bacteria engulfment were suggested to be dynamically modulated by miRNAs in either symbiotic or nonsymbiotic bacteria challenge. It was suggested that G. platifrons mussels could maintain a robust immune response against invading pathogens while establishing symbiosis with chemosynthetic bacteria with the orchestra of immune-related genes and miRNAs.
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000714697000006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177287]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Li, Chaolun
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hao,Wang, Minxiao,Zhang, Huan,et al. microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2021,119:420-431.
APA	Chen, Hao.,Wang, Minxiao.,Zhang, Huan.,Wang, Hao.,Zhou, Li.,...&Li, Chaolun.(2021).microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation.FISH & SHELLFISH IMMUNOLOGY,119,420-431.
MLA	Chen, Hao,et al."microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation".FISH & SHELLFISH IMMUNOLOGY 119(2021):420-431.