Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture

doi:10.1016/j.aquaculture.2021.736850

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture
	Yang, Na 3,4; Song, Fuhang 2; Polyak, Steven W.1; Liu, Jianguo 3,4
刊名	AQUACULTURE
	2021-08-30
卷号	541 页码:8
关键词	Aquaculture pathogen Vibrio anguillarum Antibiotic resistance mechanisms Transcriptome Respiration Actinonin
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2021.736850
通讯作者	Song, Fuhang(songfuhang@btbu.edu.cn) ; Liu, Jianguo(jgliu@qdio.ac.cn)
英文摘要	Drug-resistant bacteria are an increasingly serious threat to the aquaculture industry. In order to develop new methods to combat these bacteria, it is necessary to first understand their mechanisms of drug resistance. We previously isolated a potential probiotic strain, Streptomyces sp. NHF165, which produced cytotoxic actinonin against the aquaculture pathogen Vibrio anguillarum. In this study, a spontaneous V. anguillarum mutant with an eight-fold increased MIC for actinonin was characterized for elucidation of actinonin resistance mechanisms. Transcriptomic analysis revealed significant up-regulation of genes related to anaerobic respiratory (nap and tor) and down-regulation of genes involved in anaerobic respiration (nqr, sdh and frd) in mutant strains. Meanwhile, no mutations in bacterial respiration genes or translation initiation genes (the proposed actinonin targeted pathway) were detected by whole genome resequencing analysis. A series of experiments confirmed that actinonin-resistance in the mutant strain was associated with respiration. When aerobic respiration was chemically disrupted by CCCP, the growth of wild-type V. anguillarum changed significantly, with a 11.74-fold increase in cell density under actinonin treatment. For anaerobic respiration, an increased nitrate reductase activity was detected in mutant cells by addition of an exogenous electron donor. Noteworthy was the finding that nitrate inhibited the growth of the wild-type strain but promoted the growth of the mutant. Futhermore, the product of nitrate-dependent anaerobic respiration, nitrite, did not arrest the growth of mutant V. anguillarum, which was contrary to previous reports and might exacerbate the formation of vibriosis. Finally, we showed that chemical inhibition of nitrate reductase could restore the susceptibility of mutant V. anguillarum to actinonin. Together these data demonstrate that the combined use of an anaerobic-respiratory inhibitor and an antibiotic might be a promising approach for the treatment of drug-resistant bacteria in aquaculture.
资助项目	National Natural Science Foundation of China[31600136] ; Young Scientists Fund of LMBB, Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04] ; Shandong Provincial Natural Science Foundation, China[ZR2016CB13] ; Research Foundation for Advanced Talents of Beijing Technology and Business University[19008020158]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000657609900003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176846]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Song, Fuhang; Liu, Jianguo
作者单位	1.Univ South Australia, UniSA Clin & Hlth Sci, Adelaide, SA, Australia 2.Beijing Technol & Business Univ, Sch Light Ind, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yang, Na,Song, Fuhang,Polyak, Steven W.,et al. Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture[J]. AQUACULTURE,2021,541:8.
APA	Yang, Na,Song, Fuhang,Polyak, Steven W.,&Liu, Jianguo.(2021).Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture.AQUACULTURE,541,8.
MLA	Yang, Na,et al."Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture".AQUACULTURE 541(2021):8.