Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

doi:10.1111/are.15683

CORC > 海洋研究所 > 中国科学院海洋研究所

	Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress
	Ge, Hongxing 1,2,3,4; Shi, Junjie 3; Liu, Jialing 3; Liang, Xiafei 3; Dong, Zhiguo 3
刊名	AQUACULTURE RESEARCH
	2021-11-26
页码	15
关键词	clam metabolism miRNA molecular response mRNA NH3
ISSN号	1355-557X
DOI	10.1111/are.15683
通讯作者	Dong, Zhiguo(dzg7712@163.com)
英文摘要	Clam Cyclina sinensis behaves a stronger ammonia nitrogen tolerance, whereas the molecular regulation mechanism remains unknown. In the present study, C. sinensis was exposed to ammonia nitrogen (32.90 mg/L) for 0 h (the control), 12 h (T1) and 24 h (T2). Integrated analysis of miRNA-mRNA was conducted to reveal the regulatory roles of miRNAs in the metabolism of C. sinensis exposed to acute ammonia nitrogen. Numerous genes involved in detoxification and ammonia excretion expressed differently indicate that nitrogen assimilation and utilization is adjusted to accommodate ammonia exposure. The clam has to alter their glucose and lipid metabolism to meet energy requirements to adapt acute ammonia exposure in the environment. 12, 948 genes were identified as target genes of the differently expressed miRNAs. Most of mRNA-miRNA pairs were involved in metabolisms of carbohydrate, lipid and amino acid. miRNA-mRNA integrated analysis revealed involvement of Purine metabolism, Wnt signalling pathway, Hippo signalling pathway, PI3K-Akt signalling pathway in ammonia-stress response of C. sinensis. Exposed to ammonia, clam may suffer hypoxia exposure. Overall, exposed to ammonia, clam has to alter their glucose and lipid metabolism to meet energy requirements. Numerous mRNA-miRNA pairs were involved in metabolisms of carbohydrate, lipid and amino acid and miRNA can regulate the metabolism of C. sinensis by regulating the expression of target genes during acute ammonia nitrogen stress. The net result is that nitrogen assimilation and utilization is adjusted to accommodate ammonia exposure. The current study is helpful for further investigation into the ammonia nitrogen response mechanisms in mollusks.
资助项目	National Key Research and Development projects[2019YFD0900403] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA ; Priority and Academic Program Development of Jiangsu Higher Education Institutions[20KJB240002] ; Opening Foundation of Jiangsu Institute of Marine Resources Development[JSIMR202017]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000722748800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177272]
专题	中国科学院海洋研究所
通讯作者	Dong, Zhiguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Jiangsu Inst Marine Resources Dev, Lianyungang, Peoples R China 3.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Ecoenvironm, Lianyungang 222005, Jiangsu, Peoples R China 4.Jiangsu Inst Marine Resources Dev, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang, Peoples R China
推荐引用方式 GB/T 7714	Ge, Hongxing,Shi, Junjie,Liu, Jialing,et al. Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress[J]. AQUACULTURE RESEARCH,2021:15.
APA	Ge, Hongxing,Shi, Junjie,Liu, Jialing,Liang, Xiafei,&Dong, Zhiguo.(2021).Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress.AQUACULTURE RESEARCH,15.
MLA	Ge, Hongxing,et al."Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress".AQUACULTURE RESEARCH (2021):15.