Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota

doi:10.1021/acs.analchem.1c00962

	Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota
	Zhang, Xu 3,4; Cheng, Kai 3,4; Ning, Zhibin 3,4; Mayne, Janice 3,4; Walker, Krystal 3,4; Chi, Hao 2; Farnsworth, Charles L.1; Lee, Kimberly 1; Figeys, Daniel 3,4
刊名	ANALYTICAL CHEMISTRY
	2021-05-04
卷号	93 期号:17 页码:6594-6598
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c00962
英文摘要	Lysine acylations are important post-translational modifications that are present in both eukaryotes and prokaryotes and regulate diverse cellular functions. Our knowledge of the microbiome lysine acylation remains limited due to the lack of efficient analytical and bioinformatics methods for complex microbial communities. Here, we show that the serial enrichment using motif antibodies successfully captures peptides containing lysine acetylation, propionylation, and succinylation from human gut microbiome samples. A new bioinformatic workflow consisting of an unrestricted database search confidently identified >60,000 acetylated, and similar to 20,000 propionylated and succinylated gut microbial peptides. The characterization of these identified modification-specific metaproteomes, i.e., meta-PTMomes, demonstrates that lysine acylations are differentially distributed in microbial species with different metabolic capabilities. This study provides an analytical framework for the study of lysine acylations in the microbiome, which enables functional microbiome studies at the post-translational level.
资助项目	Government of Canada through Genome Canada ; Ontario Genomics Institute[OGI-156] ; Ontario Genomics Institute[OGI-149] ; Natural Sciences and Engineering Research Council of Canada (NSERC)[210034] ; Ontario Ministry of Economic Development and Innovation[ORF-DIG-14405] ; Ontario Ministry of Economic Development and Innovation[13440]
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000648505900004
内容类型	期刊论文
源URL	[http://119.78.100.204/handle/2XEOYT63/17689]
专题	中国科学院计算技术研究所
通讯作者	Figeys, Daniel
作者单位	1.Cell Signaling Technol Inc, Danvers, MA 01923 USA 2.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 3.Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada 4.Univ Ottawa, Fac Med, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
推荐引用方式 GB/T 7714	Zhang, Xu,Cheng, Kai,Ning, Zhibin,et al. Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota[J]. ANALYTICAL CHEMISTRY,2021,93(17):6594-6598.
APA	Zhang, Xu.,Cheng, Kai.,Ning, Zhibin.,Mayne, Janice.,Walker, Krystal.,...&Figeys, Daniel.(2021).Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota.ANALYTICAL CHEMISTRY,93(17),6594-6598.
MLA	Zhang, Xu,et al."Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota".ANALYTICAL CHEMISTRY 93.17(2021):6594-6598.