Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau

doi:10.1016/j.gecco.2021.e01937

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所 > 山地表生过程与生态调控重点实验室

	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
	Hu, Yang 3,4; Jiang, Hongmao 3,4; Chen, Youchao 2; Wang, Ziwei 3,4; Yan, Yan 4; Sun, Ping 1; Lu, Xuyang 4
刊名	GLOBAL ECOLOGY AND CONSERVATION
	2021-12-01
卷号	32 页码:12
关键词	Alpine steppe Nitrogen addition Microbial functional genes (MFGs) Q-PCR Tibetan Plateau
ISSN号	无
DOI	10.1016/j.gecco.2021.e01937
英文摘要	The microbial-mediated functional potentials of soil can be reflected by the abundance of corresponding microbial functional genes (MFGs). Few studies have simultaneously examined the responses of multiple key MFGs involving soil carbon (C) and nitrogen (N) transformation to N deposition, particularly under multiple application levels. In this study, we treated the alpine soil from the Tibetan Plateau with N fertilization at six addition rates. Absolute quantitative analysis was used to detect the abundance of MFGs related to soil C process: carbon dioxide (CO2) fixation (cbbL), methane (CH4) oxidation (pmoA) and production (mcrA), and soil N process: ammonia oxidation (AOA-amoA: archaea amoA, AOB-amoA: bacterial amoA), hydroxylamine oxidation (hao), nitrate, nitrite, nitric oxide and nitrous oxide (N2O) reduction (narG/napA, nirS/nirK, norB, and nosZ). The abundance of MFGs involved in both the soil C and N transformation processes were reduced as N rates increased (except AOB-amoA gene). Specifically, the abundance of the mcrA, AOA-amoA, nirK, norB, and nosZ genes were decreased non-linearly (U-shape) with the increasing N rates (P < 0.05). The soil pH and dissolved organic C (DOC) concentration were positively correlated with the abundance of those MFGs, suggesting that the decreases in abundance of MFGs were more likely to be driven by changes of soil pH and DOC concentration. In contrast, the abundance of AOB-amoA gene increased linearly with the increasing N rates, which was strongly and positively correlated with soil nitrate-N (NO3--N) concentration (P < 0.001, r = 0.82). In addition, soil greenhouse gases emissions linked with the abundance of corresponding MFGs. Specifically, the soil CO2 and N2O emissions were strongly and positively correlated with the AOB-amoA gene abundance (P < 0.001, r = 0.78, r = 0.84), and the soil CH4 emissions were significantly correlated with the pmoA (P < 0.01, r = 0.60) and AOA-amoA (P < 0.001, r = 0.72) gene abundance. The above results showed that the AOB-amoA gene had a positive response to increasing N rates, which was different from other genes. Indicating that elevated N deposition may improve the ammonia-oxidation process in alpine steppe soil mediated by AOB communities, and AOB communities may play an important role in donating the soil-atmosphere C and N exchange via CO2 and N2O emissions.
资助项目	National Natural Science Foundation of China[41877338] ; National Natural Science Foundation of China[41871049] ; National Natural Science Foundation of China[31901198]
WOS关键词	AMMONIA-OXIDIZING BACTERIA ; GREENHOUSE-GAS EMISSIONS ; ORGANIC-CARBON ; ARCHAEA ; COMMUNITIES ; DEPOSITION ; GRASSLAND ; ABUNDANCE ; RESPONSES ; GENES
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000734884900006
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/56383]
专题	成都山地灾害与环境研究所_山地表生过程与生态调控重点实验室
通讯作者	Lu, Xuyang
作者单位	1.Jinling Inst Technol, Nanjing 211169, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Mt Hazards & Environm, Key Lab Mt Surface Proc & Ecol Regulat, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yang,Jiang, Hongmao,Chen, Youchao,et al. Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau[J]. GLOBAL ECOLOGY AND CONSERVATION,2021,32:12.
APA	Hu, Yang.,Jiang, Hongmao.,Chen, Youchao.,Wang, Ziwei.,Yan, Yan.,...&Lu, Xuyang.(2021).Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau.GLOBAL ECOLOGY AND CONSERVATION,32,12.
MLA	Hu, Yang,et al."Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau".GLOBAL ECOLOGY AND CONSERVATION 32(2021):12.