一株促甲烷氧化假单胞菌Pseudomonas putida P7的分离及电活性特征

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	一株促甲烷氧化假单胞菌Pseudomonas putida P7的分离及电活性特征
	郝钦钦 1,2,3; 张月超 1,2,3; 李新 1,2,3; 刘芳华 1,2
刊名	微生物学报
	2020
卷号	60 期号:9 页码:2062-2071
关键词	甲烷氧化湿地电化学活性
ISSN号	0001-6209
其他题名	Isolation and electroactive characteristics of a methane-oxidation promoting Pseudomonas putida P7
文献子类	Article
英文摘要	[Objective] To analyze the physiological and electrochemical characteristics of a methanotrophs-associated bacteria isolated from the methanotrophic enrichments in the Yellow River Delta wetland soil, and to explore their effect on the methane oxidation process. [Methods] High-throughput sequencing was used to analyze microbial community of methanotrophic enrichments. The methanotrophs-associated strain was isolated using plate smearing and scribing method and initially identified by 16S rRNA gene sequencing. The morphology of the isolate was depicted by scanning electron microscopy. Gas chromatography was used to analyze the methane concentration, which showed the ability of utilizing methane and influence of improving methane oxidation by methanotroph. Two-chamber microbial fuel cells and Differential Pulse Voltammetry were used to test the electrochemical activity. [Results] The microbial community of methanotrophic enrichments from incubation of Yellow River Delta wetland soil included methanotrophs Methylobacter and other accompanying genera. We isolated a methanol-metabolizing bacterium, Pseudomonas putida P7 (with the similarity of 99.79%) which was a rod bacterium with the length between 1.5 m and 2.5 m and width about 0.5 pm. The GC analysis showed that this strain could not use methane but improved methane oxidation (P<0.05). The maximum current density was 28 mA/m~2, and the results of DPV revealed that the oxidation peak and reduction peak occurred at-0.17 V and -0.25 V,respectively. [Conclusion] We successfully isolated an electrochemical activity microbe, Pseudomonas putida P7, with ability of improving methane oxidation. This study deepened the understanding of the physiological characteristics and functions of the methanotroph-associated bacteria in the process of methane oxidation.
语种	中文
CSCD记录号	CSCD:6799318
资助机构	国家自然科学基金 ; 泰山学者青年专家计划
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/30339]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.中国科学院烟台海岸带研究所,海岸带生物学与生物资源利用重点实验室,山东烟台264003; 2.中国科学院海岸带环境过程与生态修复重点实验室(烟台海岸带研究所),山东烟台264003; 3.中国科学院大学,北京100049
推荐引用方式 GB/T 7714	郝钦钦,张月超,李新,等. 一株促甲烷氧化假单胞菌Pseudomonas putida P7的分离及电活性特征[J]. 微生物学报,2020,60(9):2062-2071.
APA	郝钦钦,张月超,李新,&刘芳华.(2020).一株促甲烷氧化假单胞菌Pseudomonas putida P7的分离及电活性特征.微生物学报,60(9),2062-2071.
MLA	郝钦钦,et al."一株促甲烷氧化假单胞菌Pseudomonas putida P7的分离及电活性特征".微生物学报 60.9(2020):2062-2071.