A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A

doi:10.1039/d0an01220c

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A
	Ding, Yangjun 1; Shang, Hezhen 2; Wang, Xiaokun 3; Chen, Lingxin 4,5
刊名	ANALYST
	2020-09-21
卷号	145 期号:18 页码:6079-6084
关键词	COLORIMETRIC APTASENSOR GOLD NANOPARTICLES TOXICITY PROBES RISK
ISSN号	0003-2654
DOI	10.1039/d0an01220c
英文摘要	Ochratoxin A (OTA) is a well-known carcinogenic contaminant in food commodities. The highly sensitive and specific detection of the OTA level is an essential way to prevent the health risks to humans/animals. In this work, an SERS-based competitive immunoassay platform has been developed for the detection of OTA. This assay contains two compartments: OTA-BSA-immobilized SERS nanotags and anti-OTA antibody-functionalized magnetic beads. In the presence of target OTA, a competitive reaction towards magnetic beads occurs between the target OTA and SERS nanotags. The characteristic peaks of SERS nanotags adsorbed onto magnetic beads were used for the quantitative analysis of OTA. Under optimized conditions, a good linear relationship was obtained in the range of 1 pg mL(-1)to1000 pg mL(-1). The limit of detection (LOD) was estimated to be 0.61 pg mL(-1)using the IUPAC standard method. In addition, this proposed method was also successfully applied for the detection of OTA in wine samples and satisfactory recovery rates were obtained in the range of 90.60% to 103.3%. It is believed that this SERS-based competitive method holds great potential for highly sensitive and specific detection of mycotoxins.
WOS关键词	COLORIMETRIC APTASENSOR ; GOLD ; NANOPARTICLES ; TOXICITY ; PROBES ; RISK
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000568961600009
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21904078, 21976209] ; Taishan Scholar Project Special Funding [ts20190962]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/30492]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China; 2.Qingdao Chengyang Dist Peoples Hosp, Dept Hepatobiliary Surg, Qingdao 266109, Peoples R China; 3.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China; 4.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Ding, Yangjun,Shang, Hezhen,Wang, Xiaokun,et al. A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A[J]. ANALYST,2020,145(18):6079-6084.
APA	Ding, Yangjun,Shang, Hezhen,Wang, Xiaokun,&Chen, Lingxin.(2020).A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A.ANALYST,145(18),6079-6084.
MLA	Ding, Yangjun,et al."A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A".ANALYST 145.18(2020):6079-6084.