An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells

doi:10.1016/j.tiv.2019.104667

	An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells
	Wang, Chun-Ming 1; Huo, Xiang 1; Chen, Jun 1; Liu, Jia-Wei 1; Yang, Ting-Yu 1; Mi, Xiang-Quan 1; Meng, Yue 1; Zhou, Li 1; Lin, Chang-Jun 1; Liu, Jing 2
刊名	TOXICOLOGY IN VITRO
	2020-02-01
卷号	62 页码:7
关键词	Xanthohumol Reactive oxygen species Pro-oxidant Acute lytic cell death Cell rupture
ISSN号	0887-2333
DOI	10.1016/j.tiv.2019.104667
通讯作者	Wang, Chun-Ming(cmwang@lzu.edu.cn) ; Liu, Jing(liuj@impcas.ac.cn)
英文摘要	Serum is an important component in cell culture medium. It also possesses potent antioxidant properties. Therefore, the conventional protocols for detecting reactive oxygen species (ROS) in cultured cells with fluorescent probes include washing and suspending cells with serum-free buffers, such as PBS. This transient serum deprivation is essential for the ROS detecting. Unfortunately, it may also cause unexpected results, which push us to choose more optimal experiment conditions. In the present study, we found an acute lytic cell death induced by xanthohumol (XN), which obstructed ROS detecting in human leukemia cell line HL-60 cells. XN induced ROS burst, caused cell swelling, membrane permeability increase, LDH release, and ultimately an acute lyric cell death and cell rupture. These effects could be alleviated by the antioxidant N-Acetyl-L-cysteine (NAC). Apoptosis, pyroptosis or necroptosis were not observed in this process. Results also indicated that 2% serum addition had already completely scavenged ROS induced by 10 mu M XN. Taken together, it is strongly suggested to detecting ROS in a serum-free medium when studying where and how ROS generated in cells. The concentration at the ROS maximum point (10 mu M XN in this study) can be selected as the optimal concentration.
资助项目	National Natural Science Foundation of China[31071917] ; Western Light Foundation ; Technology to support Gansu Province Foundation of Chinese Academy of Sciences[KJZG-2011-002]
WOS关键词	PHARMACOLOGICAL PROFILE ; ANTIOXIDANT PROPERTIES ; APOPTOSIS ; GENERATION ; HOPS
WOS研究方向	Toxicology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000500376200001
资助机构	National Natural Science Foundation of China ; Western Light Foundation ; Technology to support Gansu Province Foundation of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141241]
专题	中国科学院近代物理研究所
通讯作者	Wang, Chun-Ming; Liu, Jing
作者单位	1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chun-Ming,Huo, Xiang,Chen, Jun,et al. An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells[J]. TOXICOLOGY IN VITRO,2020,62:7.
APA	Wang, Chun-Ming.,Huo, Xiang.,Chen, Jun.,Liu, Jia-Wei.,Yang, Ting-Yu.,...&Liu, Jing.(2020).An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells.TOXICOLOGY IN VITRO,62,7.
MLA	Wang, Chun-Ming,et al."An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells".TOXICOLOGY IN VITRO 62(2020):7.