Novel mutagenesis and screening technologies for food microorganisms: advances and prospects

doi:10.1007/s00253-019-10341-z

	Novel mutagenesis and screening technologies for food microorganisms: advances and prospects
	Yu, Qinghui 1,2; Li, Yuncheng 1; Wu, Bo 2; Hu, Wei 3; He, Mingxiong 2; Hu, Guoquan 2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2020-02-01
卷号	104 期号:4 页码:1517-1531
关键词	Strain development Food microorganism Mutagenesis Screening Microscale cultivation
ISSN号	0175-7598
DOI	10.1007/s00253-019-10341-z
通讯作者	Wu, Bo(wubo@caas.cn) ; Hu, Wei(hudongli007@impcas.ac.cn)
英文摘要	Microorganisms are indispensable in the food industry, but wild-type strains hardly meet the current industrial demands due to several undesirable traits. Therefore, microbial strain improvement offers a critical solution to enhance the food industry. Traditional techniques for food microbial improvement, such as the use of chemical mutagens and manual isolation/purification, are inefficient, time-consuming, and laborious, restricting further progress in the area of food fermentation. In this review, the applications of novel mutagenesis and screening technologies used for the improvement of food microbes were summarized, including random mutagenesis based on physical irradiation, microbial screening facilitated by a microtiter plate, fluorescence-activated cell or droplet sorting, and microscaled fermentation in a microtiter plate or microbioreactor. In comparison with conventional methods, these new tools have the potential in accelerating microbial strain improvement and their combined applications could create a new trend for strain development. However, several problems that could affect its potential application may include the following: the lack of specific mutagenesis devices and biosensing systems, the insufficient improvement of the mixed culture system, the low efficiency when using filamentous fungi and flocculating bacteria, and the insufficient safety assessment on harnessing genome-editing technology. Therefore, future works on strain improvement remain challenging for the food industry.
WOS关键词	TEMPERATURE PLASMA ARTP ; L-LACTIC ACID ; SACCHAROMYCES-CEREVISIAE ; MICROTITER PLATES ; BACILLUS-AMYLOLIQUEFACIENS ; ENHANCED PRODUCTION ; ASPERGILLUS-NIGER ; MEAL HYDROLYSATE ; OXYGEN-TRANSFER ; FLOW-CYTOMETRY
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000519153800011
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/140909]
专题	中国科学院近代物理研究所
通讯作者	Wu, Bo; Hu, Wei
作者单位	1.Chengdu Univ, Coll Pharm & Biol Engn, 2025 Chengluo Ave, Chengdu 610106, Peoples R China 2.Minist Agr & Rural Affairs, Biogas Inst, Biomass Energy Technol Res Ctr, 4-13 Rennin Rd, Chengdu 610041, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Yu, Qinghui,Li, Yuncheng,Wu, Bo,et al. Novel mutagenesis and screening technologies for food microorganisms: advances and prospects[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(4):1517-1531.
APA	Yu, Qinghui,Li, Yuncheng,Wu, Bo,Hu, Wei,He, Mingxiong,&Hu, Guoquan.(2020).Novel mutagenesis and screening technologies for food microorganisms: advances and prospects.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(4),1517-1531.
MLA	Yu, Qinghui,et al."Novel mutagenesis and screening technologies for food microorganisms: advances and prospects".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.4(2020):1517-1531.