The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks

doi:10.1016/j.indcrop.2020.112159

	The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks
	Tian, Xuejiao 2,3; Hu, Wei 2,3; Chen, Jihong 2,3; Zhang, Wei 1; Li, Wenjian 2,3
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2020-04-01
卷号	146 页码:7
关键词	Vitamin C L-lactic acid Sweet sorghum juice Antioxidant capacity Fermentation efficiency
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2020.112159
通讯作者	Hu, Wei(hudongli007@impcas.ac.cn)
英文摘要	Regulating oxidative damage is an effective way to enhance cell growth and metabolite accumulation in microbial strains. However, this strategy on lactic acid production has not been systematically reported yet. In present study, the effect of antioxidant vitamin C on cell growth and L-lactic acid production was investigated in Lactobacillus thermophilus A69, and an addition of 75 mM vitamin C resulted in significant improvement in cell growth and L-lactic acid accumulation. The total antioxidant capacity (T-AOC) and key enzyme activities involving in glycolytic pathway (such as hexokinase, phosphofructokinase, pyruvate kinase, and lactate dehydrogenase) were also significantly enhanced by vitamin C addition. Finally, with the addition of optimal concentrations of vitamin C into sweet sorghum juice coupled with cheaper soybean hydrolysate as feedstocks, a final L-lactic acid titer of 118.8 g/L with the productivity of 3.71 g/L/h was achieved by A69 strain. This study provided an efficient and economical approach for enhancement of L-lactic acid fermentation efficiency using sweet sorghum juice as a feedstock.
资助项目	National Natural Science Foundation of China[U1932136] ; Western Talents Program of the Chinese Academy of Sciences[Y706030XB0] ; Open Funds of Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province[EEMRE201801]
WOS关键词	REPEATED-BATCH FERMENTATION ; BACILLUS-COAGULANS ; NITROGEN-SOURCE ; DOCOSAHEXAENOIC ACID ; ENHANCED PRODUCTION ; RHAMNOSUS ; OPTIMIZATION ; PRODUCTIVITY ; ANTIOXIDANT ; IMPROVEMENT
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000519335100028
资助机构	National Natural Science Foundation of China ; Western Talents Program of the Chinese Academy of Sciences ; Open Funds of Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/140596]
专题	中国科学院近代物理研究所
通讯作者	Hu, Wei
作者单位	1.Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tian, Xuejiao,Hu, Wei,Chen, Jihong,et al. The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,146:7.
APA	Tian, Xuejiao,Hu, Wei,Chen, Jihong,Zhang, Wei,&Li, Wenjian.(2020).The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks.INDUSTRIAL CROPS AND PRODUCTS,146,7.
MLA	Tian, Xuejiao,et al."The supplement of vitamin C facilitates L-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks".INDUSTRIAL CROPS AND PRODUCTS 146(2020):7.