Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice

doi:10.1177/1559325820951332

	Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice
	Liu, Cuihua 1; Tanaka, Kaoru 1; Katsube, Takanori 1; Vares, Guillaume 2; Maruyama, Kouichi 1; Ninomiya, Yasuharu 1; Fardous, Zeenath 3; Sun, Chao 4; Fujimori, Akira 1; Moreno, Stephanie G.5
刊名	DOSE-RESPONSE
	2020-07-01
卷号	18 期号:3 页码:11
关键词	green fluorescent protein apoptosis bone marrow death adaptive response ionizing radiation GFP transgenic mice
ISSN号	1559-3258
DOI	10.1177/1559325820951332
通讯作者	Nenoi, Mitsuru(nenoi.mitsuru@qst.go.jp) ; Wang, Bing(wang.bing@qst.go.jp)
英文摘要	Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice.
资助项目	Ministry of Education, Culture, Sport, Sciences and Technology (MEXT), Japan ; Nuclear Safety Research Association, Japan ; JSPS KAKENHI, Japan[25340041]
WOS关键词	GREEN FLUORESCENT PROTEIN ; TRANSPLANTED PANCREATIC-ISLETS ; MARROW-DERIVED CELLS ; OXIDATIVE STRESS ; IN-VIVO ; RADIOADAPTIVE RESPONSE ; TRANSGENIC EXPRESSION ; ADAPTIVE RESPONSE ; GERM-CELLS ; GFP
WOS研究方向	Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology
语种	英语
出版者	SAGE PUBLICATIONS INC
WOS记录号	WOS:000566468700001
资助机构	Ministry of Education, Culture, Sport, Sciences and Technology (MEXT), Japan ; Nuclear Safety Research Association, Japan ; JSPS KAKENHI, Japan
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/139804]
专题	中国科学院近代物理研究所
通讯作者	Nenoi, Mitsuru; Wang, Bing
作者单位	1.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Chiba, Japan 2.Okinawa Inst Sci & Technol, Cell Signal Unit, Okinawa, Japan 3.Bangladesh Atom Energy Commiss, Inst Food & Radiat Biol, Atom Energy Res Estab, Dhaka, Bangladesh 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 5.INSERM, Fundamental Res Div, LRTS Francois Jacob Inst Biol, Atom Energy & Alternat Energies Commiss, Fontenay Aux Roses, France 6.Natl Inst Quantum & Radiol Sci & Technol, Dept Safety Adm, Chiba, Japan
推荐引用方式 GB/T 7714	Liu, Cuihua,Tanaka, Kaoru,Katsube, Takanori,et al. Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice[J]. DOSE-RESPONSE,2020,18(3):11.
APA	Liu, Cuihua.,Tanaka, Kaoru.,Katsube, Takanori.,Vares, Guillaume.,Maruyama, Kouichi.,...&Wang, Bing.(2020).Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice.DOSE-RESPONSE,18(3),11.
MLA	Liu, Cuihua,et al."Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice".DOSE-RESPONSE 18.3(2020):11.