Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells

doi:10.1186/s12935-021-02268-1

	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
	Zhao,Xueshan 1,5; Jin,Xiaodong 2; Zhang,Qiuning 2,3,4; Liu,Ruifeng 2,3,4; Luo,Hongtao 2,3,4; Yang,Zhen 2; Geng,Yichao 1; Feng,Shuangwu 1; Li,Chengcheng 1; Wang,Lina 1
刊名	Cancer Cell International
	2021-12-04
卷号	21 期号:1 页码:12
关键词	LncRNA H19 MiR-130a-3p WNK3 Non-small-cell lung cancer Radiotherapy
DOI	10.1186/s12935-021-02268-1
通讯作者	Wang,Xiaohu(xhwang@impcas.ac.cn) ; Li,Qiang(liqiang@impcas.ac.cn)
英文摘要	AbstractBackgroundThe lncRNA H19 is believed to act as an oncogene in various types of tumors and is considered to be a therapeutic target and diagnostic marker. However, the role of the lncRNA H19 in regulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells is unknown.MethodsThe expression profiles of lncRNAs in NSCLC were explored via transcriptome sequencing. CCK-8, EdU incorporation and clonogenic survival assays were conducted to evaluate the proliferation and radiosensitivity of NSCLC cells. Flow cytometry and Western blotting were conducted to measure the level of apoptosis. The binding relationship between the lncRNA H19 and miR-130a-3p was determined by a dual-luciferase reporter assay. A binding relationship was also identified between miR-130a-3p and With-No-Lysine Kinase 3 (WNK3).ResultsExpression?patterns of lncRNAs revealed that the lncRNA H19 was upregulated in radioresistant NSCLC (A549-R11) cells compared with A549 cells. Knockdown of the lncRNA H19 enhanced the sensitivity?of NSCLC cell lines to X-ray and carbon ion irradiation. Mechanistically, the lncRNA H19 serves as a sponge of miR-130a-3p, which downregulates WNK3 expression. The lncRNA H19–miR-130a-3p–WNK3 axis modulates radiosensitivity by regulating apoptosis in NSCLC cell lines.ConclusionKnockdown of the lncRNA H19 promotes the sensitivity of NSCLC cells to X-ray and carbon ion irradiation. Hence, the lncRNA H19 might function as a potential therapeutic target that enhances the antitumor effects of radiotherapy in NSCLC.
资助项目	Science and Technology Plan Project of Chengguan District of Lanzhou[2020-2-2-5] ; Talent innovation and venture project of Lanzhou city[2020-RC-113] ; Talent innovation and venture project of Lanzhou city[2017RC-23] ; Lanzhou KejinTaiji Corporation, Ltd[BMP-B-02-002] ; Key Deployment Project of Chinese Academy of Sciences[KFZD-SW-222]
WOS关键词	P38 MAPK INHIBITOR ; NONCODING RNA H19 ; LUNG-CANCER ; PRELIMINARY EFFICACY ; DOSE-ESCALATION ; PHASE 1/2A ; EXPRESSION ; BC-819 ; RALIMETINIB ; THERAPY
WOS研究方向	Oncology
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12935-021-02268-1
资助机构	Science and Technology Plan Project of Chengguan District of Lanzhou ; Talent innovation and venture project of Lanzhou city ; Lanzhou KejinTaiji Corporation, Ltd ; Key Deployment Project of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/138257]
专题	中国科学院近代物理研究所
通讯作者	Wang,Xiaohu; Li,Qiang
作者单位	1.Lanzhou University; The First School of Clinical Medicine 2.Chinese Academy of Sciences; Institute of Modern Physics 3.University of Chinese Academy of Sciences 4.Lanzhou Heavy Ion Hospital 5.Affiliated Hospital of Qinghai University
推荐引用方式 GB/T 7714	Zhao,Xueshan,Jin,Xiaodong,Zhang,Qiuning,et al. Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells[J]. Cancer Cell International,2021,21(1):12.
APA	Zhao,Xueshan.,Jin,Xiaodong.,Zhang,Qiuning.,Liu,Ruifeng.,Luo,Hongtao.,...&Li,Qiang.(2021).Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells.Cancer Cell International,21(1),12.
MLA	Zhao,Xueshan,et al."Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells".Cancer Cell International 21.1(2021):12.