Osmotic stress-triggered stomatal closure requires Phospholipase D delta and hydrogen sulfide in Arabidopsis thaliana

doi:10.1016/j.bbrc.2020.10.074

	Osmotic stress-triggered stomatal closure requires Phospholipase D delta and hydrogen sulfide in Arabidopsis thaliana
	Liu, Qin 2; Zhou, Yaping 2; Li, Hui 2; Liu, Ruirui 2; Wang, Wei 2; Wu, Wangze 2; Yang, Ning 2; Wang, Shuyang 1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2021
卷号	534 页码:914-920
关键词	Osmotic stress PLD delta H2S Stomatal closure Arabidopsis thaliana
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2020.10.074
通讯作者	Yang, Ning(xbsd-yn@163.com)
英文摘要	Osmotic stress is one of the main stresses seriously affects the growth and development of plants. Hydrogen sulfide (H2S) emerges as the third gaseous signal molecule to involve in the complex network of signaling events. Phospholipase D delta (PLD delta), as signal enzyme, responds to many biotic or abiotic stress responses. In this study, the functions and the relationship of PLD delta and H2S in stomatal closure induced by osmotic stress were explored. Using the seedlings of ecotype (WT), PLD delta deficient mutant (pld delta), L-cysteine desulfhydrase (LCD) deficient mutant (lcd) and pld delta lcd double mutant as materials, the Realtime quantitative PCR (RT-qPCR) and the stomatal aperture were analyzed. Osmotic stress induced the expressions of PLD delta and LCD. The H2S content and the activities of PLD and LCD ascended in WT under osmotic stress. The phenotypes of pld delta, lcd and pld delta lcd were more sensitive to osmotic stress than WT. Compared with pld delta, the stomatal of lcd showed lower sensitivity to osmotic stress, and the stomatal aperture of pld delta lcd was similar to that of lcd. Simultaneous application of PA and NaHS resulted in tighter closure of stomatal than application of either PA or NaHS alone. These results suggested that osmotic stress-triggered stomatal closure requires PLD delta and H2S in A. thaliana. LCD acted downstream of PLD delta to regulate the stomatal closure induced by osmotic stress. (c) 2020 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of China[31660116] ; National Natural Science Foundation of China[31960061] ; foundation of the Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations[lzujbky-2018-kb05]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000614555200007
资助机构	National Natural Science Foundation of China ; foundation of the Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/138014]
专题	中国科学院近代物理研究所
通讯作者	Yang, Ning
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qin,Zhou, Yaping,Li, Hui,et al. Osmotic stress-triggered stomatal closure requires Phospholipase D delta and hydrogen sulfide in Arabidopsis thaliana[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2021,534:914-920.
APA	Liu, Qin.,Zhou, Yaping.,Li, Hui.,Liu, Ruirui.,Wang, Wei.,...&Wang, Shuyang.(2021).Osmotic stress-triggered stomatal closure requires Phospholipase D delta and hydrogen sulfide in Arabidopsis thaliana.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,534,914-920.
MLA	Liu, Qin,et al."Osmotic stress-triggered stomatal closure requires Phospholipase D delta and hydrogen sulfide in Arabidopsis thaliana".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 534(2021):914-920.