Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study

doi:10.2147/IJN.S313678

	Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study
	Zhang, Pengcheng 1,2,3,4,5; Yu, Boyi 1,2,3; Jin, Xiaodong 1,2,3; Zhao, Ting 2,3; Ye, Fei 1,2,3; Liu, Xiongxiong 1,2,3; Li, Ping 1,2,3; Zheng, Xiaogang 2,3; Chen, Weiqiang 1,2,3; Li, Qiang 1,2,3
刊名	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2021
卷号	16 页码:4661-4674
关键词	gold nanoparticles radiosentization carbon ion irradiation tumor-bearing mice apoptosis
ISSN号	1178-2013
DOI	10.2147/IJN.S313678
通讯作者	Chen, Weiqiang(chenwq7315@impcas.ac.cn) ; Li, Qiang(liqiang@impcas.ac.cn)
英文摘要	Purpose: Gold nanoparticles (AuNPs) are widely studied as radiosensitizers, but their radio-sensitization in carbon ion radiotherapy is unsatisfactory. There is a lack of in vivo data on the radiosensitization of AuNPs under carbon ion irradiation. This study focused on the radiosensitization effect of AuNPs in the mouse melanoma cell line B16-F10 in vitro and in vivo. Materials and Methods: 11-mercaptoundecanoic acid (11-MUA)-coated gold (Au) nanoparticles (mAuNPs) formulations were prepared and characterized. To verify the radiosensitization effect of mAuNPs, hydroxyl radicals were generated in aqueous solution, and the detection of intracellular reactive oxygen species (ROS) and clone survival were carried out in vitro. The tumor growth rate (TGR) and survival of mice were analyzed to verify the radiosensitization effect of mAuNPs in vivo. The apoptosis of tumor cells was detected, and the expression of key proteins in the apoptosis pathway was verified by immunohistochemistry. Results: The intracellular ROS level in B16-F10 cells was enhanced by mAuNPs under carbon ion irradiation. The sensitization rate of mAuNPs was 1.22 with a 10% cell survival rate. Compared with irradiation alone, the inhibitory effect of mAuNPs combined with carbon ion irradiation on tumor growth was 1.94-fold higher, the survival time of mice was prolonged by 1.75-fold, and the number of apoptotic cells was increased by 1.43-fold. The ratio of key proteins Bax and Bcl2 in the apoptosis pathway was up-regulated, and the expression of caspase-3, a key executor of the apoptosis pathway, was up-regulated. Conclusion: In in vivo and in vitro experiments, mAuNPs showed radiosensitivity to carbon ion irradiation. The sensitization effect of mAuNPs on mice tumor may be achieved by activating the mitochondrial apoptosis pathway and increasing tumor tissue apoptosis. To our best knowledge, the present study is the first in vivo evidence for radiosensitization of mAuNPs in tumor-bearing mice exposed to carbon ion irradiation.
资助项目	Key Deployment Project of Chinese Academy of Sciences[KFZD-SW-222] ; National Natural Science Foundation of China[11875299] ; Key R&D Program of Gansu Provincial Department of Science and Technology[20YF3FA028] ; Gansu Health Industry Scientific Research Project[GSWSKY-2019-79] ; In-hospital Funded Project of Lanzhou University First Hospital[ldyyyn 2018-21] ; Science and Technology Project of Chengguan District, Lanzhou City[20202-2-6]
WOS关键词	CHARGED-PARTICLE THERAPY ; RADIOTHERAPY ; CELLS ; COMBINATION ; CITRATE ; LIGAND ; SIZE
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
语种	英语
出版者	DOVE MEDICAL PRESS LTD
WOS记录号	WOS:000670283300005
资助机构	Key Deployment Project of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Key R&D Program of Gansu Provincial Department of Science and Technology ; Gansu Health Industry Scientific Research Project ; In-hospital Funded Project of Lanzhou University First Hospital ; Science and Technology Project of Chengguan District, Lanzhou City
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/137041]
专题	中国科学院近代物理研究所
通讯作者	Chen, Weiqiang; Li, Qiang
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Basic Res Heavy Ion Radiat Applicat Med, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 4.Lanzhou Univ, Dept Radiat Oncol, Hosp 1, Lanzhou 730000, Peoples R China 5.Lanzhou Univ, Hosp 1, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Pengcheng,Yu, Boyi,Jin, Xiaodong,et al. Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2021,16:4661-4674.
APA	Zhang, Pengcheng.,Yu, Boyi.,Jin, Xiaodong.,Zhao, Ting.,Ye, Fei.,...&Li, Qiang.(2021).Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study.INTERNATIONAL JOURNAL OF NANOMEDICINE,16,4661-4674.
MLA	Zhang, Pengcheng,et al."Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study".INTERNATIONAL JOURNAL OF NANOMEDICINE 16(2021):4661-4674.