Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)

doi:10.1016/j.scienta.2021.110300

	Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)
	Liu, Ruiyuan; Dong, Xicun; Zhang, Miaomiao; Qu, Ying; Jin, Wenjie; Li, Xin; Zhou, Libin; Yu, Lixia; Li, Wenjian
刊名	SCIENTIA HORTICULTURAE
	2021-10-15
卷号	288 页码:17
关键词	Green wandering Jew Leaf-color mutant Temperature Transcriptome Flavonoids Photosynthesis
ISSN号	0304-4238
DOI	10.1016/j.scienta.2021.110300
通讯作者	Li, Wenjian(wjli@impcas.ac.cn)
英文摘要	Leaf color is considered a valuable trait when breeding ornamental plants. In this study, a novel thermo-sensitive leaf-color mutant (mt) of green wandering Jew was obtained through carbon-ion beam irradiation. Young leaves of the mt plant exhibited pink coloration under low temperature (mt_7 degrees C) and green coloration under normal temperature (mt_25 degrees C). However, young leaves of the wild type (WT) remained green under low and normal temperatures. Here, we compared the mt and WT in terms of their anatomical, physiological, transcriptomic, and metabolomic characteristics at 7 degrees C and 25 degrees C. Histological analyses showed that the upper epidermis and palisade parenchyma of mt were fewer and smaller. Chloroplasts of mt_25 degrees C were abnormally inflated, and leucoplasts were present instead of chloroplasts in mt_7 degrees C leaves. Physiological experiments also showed that mt_7 degrees C had lower chlorophyll and carotenoid contents, and higher anthocyanin contents; however, the chlorophyll content in mt_25 degrees C leaves were higher than that in the WT. Transcriptome profiling revealed that 99 differentially expressed genes (DEGs) and 1597 transcription factors (TFs) are involved in photosynthesis, chlorophyll biosynthesis, and flavonoid and anthocyanin biosyntheses. This study identified 8 candidate genes associated with the PS II reaction center, 13 genes related to PS I, 15 genes for LHC, 4 genes for PET/Cyt b6/f, and 2 genes for ATP synthase; these genes were differentially expressed at 7 degrees C. DEGs related to photosynthesis and chlorophyll biosynthesis were down-regulated in the pink leaves of mt_7 degrees C, whereas DEGs involved in flavonoid and anthocyanin biosyntheses were up-regulated in mt_7 degrees C and mt_25 degrees C leaves. Differential expression levels were further confirmed by qRT-PCR, Chlorophyll fluorescence signals, and previous HPLC results the. The anthocyanin content of mt_7 degrees C was 20-fold higher than that of the other pigments resulting in pink coloration at low temperature. This study provides a systematic evaluation of thermo-sensitive color variation in the mt. Based on these results, we identified key pathways related to thermo-sensitive leaf-color change in green wandering Jew.
资助项目	CAS Light of West China Program[Y806030] ; CAS Science and Technology Service Network Initiative Program[KFJ-STS-QYZD-197] ; National High Technology Research and Development Program of China[Y606080KJ1]
WOS关键词	FLUORESCENCE PARAMETERS ; CHLOROPHYLL ; PHOTOSYNTHESIS ; ARABIDOPSIS ; BIOSYNTHESIS ; STRESS ; IDENTIFICATION ; MUTATION ; PLANTS ; IRRADIATION
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000681069900001
资助机构	CAS Light of West China Program ; CAS Science and Technology Service Network Initiative Program ; National High Technology Research and Development Program of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/136690]
专题	中国科学院近代物理研究所
通讯作者	Li, Wenjian
作者单位	Chinese Acad Sci, Inst Modern Phys, Dept Biophys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ruiyuan,Dong, Xicun,Zhang, Miaomiao,et al. Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)[J]. SCIENTIA HORTICULTURAE,2021,288:17.
APA	Liu, Ruiyuan.,Dong, Xicun.,Zhang, Miaomiao.,Qu, Ying.,Jin, Wenjie.,...&Li, Wenjian.(2021).Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis).SCIENTIA HORTICULTURAE,288,17.
MLA	Liu, Ruiyuan,et al."Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)".SCIENTIA HORTICULTURAE 288(2021):17.