Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza

doi:10.12705/663.3

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza
	Li, Bin 2; Cui, Guanghong 1; Shen, Guoan 2; Zhan, Zhilai 1; Huang, Luqi 1; Chen, Jiachun ; Qi, Xiaoquan 2
刊名	SCIENTIFIC REPORTS
	2017
卷号	7
ISSN号	2045-2322
DOI	10.12705/663.3
文献子类	Article
英文摘要	CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/ Cas9 to precisely knock out the committed diterpene synthase gene ( SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacological activities, such as vasorelaxation, protection against ischemia-reperfusion injury, and antiarrhythmic effects. Three homozygous and eight chimeric mutants were obtained from 26 independent transgenic hairy root lines by Agrobacterium rhizogenes-mediated transformation. The metabolomic analysis based on LC-qTOF-MS and Q-TRAP-LC-MS/MS revealed that tanshinones, especially cryptotanshinone, tanshinone IIA and tanshinone I, are completely missing in homozygous mutants, without influencing other phenolic acid metabolites. By contrast, tanshinones are decreased but still detectable in chimeric mutants, which is similar to a previously-reported an RNAi study of SmCPS1. These results demonstrate that Agrobacterium rhizogenes-mediated transformation using CRISPR/Cas9 is a simple and efficient genome editing tool in S. miltiorrhiza, thus paving the way for large-scale genome editing in S. miltiorrhiza, which is important for pathway elucidation of secondary metabolites, quality improvement, and yield increases for this valuable traditional Chinese medicinal herb.
学科主题	Plant Sciences ; Evolutionary Biology
出版地	LONDON
WOS关键词	AGROBACTERIUM-RHIZOGENES ; HAIRY ROOT ; BIOSYNTHESIS ; TANSHINONES ; EXPRESSION ; BACTERIA ; PLASMID ; PATHWAY ; ARCHAEA ; SYSTEMS
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000405738400003
资助机构	Key Project of Chinese National Programs for Fundamental Research and Development [2013CB127000] ; Chinese National Programs for High Technology Research and Development [2012AA10A304]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22367]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China 3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Li, Bin,Cui, Guanghong,Shen, Guoan,et al. Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza[J]. SCIENTIFIC REPORTS,2017,7.
APA	Li, Bin.,Cui, Guanghong.,Shen, Guoan.,Zhan, Zhilai.,Huang, Luqi.,...&Qi, Xiaoquan.(2017).Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza.SCIENTIFIC REPORTS,7.
MLA	Li, Bin,et al."Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza".SCIENTIFIC REPORTS 7(2017).