Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach

doi:10.1016/j.scib.2017.09.010

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	Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach
	Li, Mingzhuo ; Li, Yanzhi ; Guo, Lili 5; Gong, Niandi 5; Pang, Yongzheng 4; Jiang, Wenbo 4; Liu, Yajun 5; Jiang, Xiaolan ; Zhao, Lei 2; Wang, Yunsheng 5
刊名	FRONTIERS IN PLANT SCIENCE
	2017
卷号	8
关键词	tea Camellia sinensis R2R3-MYB transcription factor phenylpropanoids shikimate pathway
ISSN号	1664-462X
DOI	10.1016/j.scib.2017.09.010
文献子类	Article
英文摘要	Green tea (Camellia sinensis, Cs) abundantly produces a diverse array of phenylpropanoid compounds benefiting human health. To date, the regulation of the phenylpropanoid biosynthesis in tea remains to be investigated. Here, we report a cDNA isolated from leaf tissues, which encodes a R2R3-MYB transcription factor. Amino acid sequence alignment and phylogenetic analysis indicate that it is a member of the MYB4-subgroup and named as CsMYB4a. Transcriptional and metabolic analyses show that the expression profile of CsMYB4a is negatively correlated to the accumulation of six flavan-3-ols and other phenolic acids. GFP fusion analysis shows CsMYB4a's localization in the nucleus. Promoters of five tea phenylpropanoid pathway genes are isolated and characterized to contain four types of AC-elements, which are targets of MYB4 members. Interaction of CsMYB4a and five promoters shows that CsMYB4a decreases all five promoters' activity. To further characterize its function, CsMYB4a is overexpressed in tobacco plants. The resulting transgenic plants show dwarf, shrinking and yellowish leaf, and early senescence phenotypes. A further genome-wide transcriptomic analysis reveals that the expression levels of 20 tobacco genes involved in the shikimate and the phenylpropanoid pathways are significantly downregulated in transgenic tobacco plants. UPLC-MS and HPLC based metabolic profiling reveals significant reduction of total lignin content, rutin, chlorogenic acid, and phenylalanine in CsMYB4a transgenic tobacco plants. Promoter sequence analysis of the 20 tobacco genes characterizes four types of AC-elements. Further CsMYB4a-AC element and CsMYB4a-promoter interaction analyses indicate that the negative regulation of CsMYB4a on the shikimate and phenylpropanoid pathways in tobacco is via reducing promoter activity. Taken together, all data indicate that CsMYB4a negatively regulates the phenylpropanoid and shikimate pathways. Highlight: A tea (Camellia sinensis) MYB4a is characterized to encode a R2R3-MYB transcription factor. It is shown to repressively control the phenylpropanoid and shikimate pathway.
学科主题	Science & Technology - Other Topics
出版地	LAUSANNE
WOS关键词	CINNAMYL ALCOHOL-DEHYDROGENASE ; R2R3-MYB GENE FAMILY ; GREEN TEA ; ARABIDOPSIS-THALIANA ; ANTHOCYANIN BIOSYNTHESIS ; LIGNIN BIOSYNTHESIS ; TRANSGENIC TOBACCO ; DOWN-REGULATION ; DISEASE ; PROTEIN
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000416210600002
资助机构	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570694, 31470689, 31300577] ; Specialized Research Fund for the Doctoral Program of Higher EducationSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20133418130001] ; Special Foundation for Independent Innovation of Anhui Province, China [13Z03012] ; Anhui Major Demonstration Project for Leading Talent Team on Tea Chemistry and Health
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22275]
专题	中科院北方资源植物重点实验室
作者单位	1.North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA 2.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 3.Qingdao Agr Univ, Coll Hort, Qingdao Key Lab Genet Improvement & Breeding Hort, Qingdao, Peoples R China 4.Anhui Agr Univ, Sch Life Sci, Hefei, Peoples R China 5.Anhui Agr Univ, State Key Lab Tea Plant Biochem & Utilizat, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Li, Mingzhuo,Li, Yanzhi,Guo, Lili,et al. Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Li, Mingzhuo.,Li, Yanzhi.,Guo, Lili.,Gong, Niandi.,Pang, Yongzheng.,...&Xia, Tao.(2017).Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach.FRONTIERS IN PLANT SCIENCE,8.
MLA	Li, Mingzhuo,et al."Functional Characterization of Tea (Camellia sinensis) MYB4a Transcription Factor Using an Integrative Approach".FRONTIERS IN PLANT SCIENCE 8(2017).