Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock

doi:10.1104/pp.19.01120

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	Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock
	Kim, Tae-Sung ; Wang, Lei 1; Kim, Yeon Jeong ; Somers, David E.
刊名	PLANT PHYSIOLOGY
	2020
卷号	182 期号:2 页码:1130-1141
ISSN号	0032-0889
DOI	10.1104/pp.19.01120
文献子类	Article
英文摘要	Circadian systems share the three properties of entrainment, free-running period, and temperature compensation (TC). TC ensures nearly the same period over a broad range of physiologically relevant temperatures; however, the mechanisms behind TC remain poorly understood. Here, we identify single point mutations in two key elements of the Arabidopsis circadian clock, GIGANTEA (GI) and ZEITLUPE (ZTL), which likely act as compensatory substitutions to establish a remarkably constant free-running period over a wide range of temperatures. Using near-isogenic lines generated from the introgression of the Cape Verde Islands (Cvi) alleles of GI and ZTL into the Landsberg erecta (Ler) background, we show how longer periods in the Cvi background at higher temperatures correlate with a difference in strength of the GI/ZTL interaction. Pairwise interaction testing of all GI/ZTL allelic combinations shows similar affinities for isogenic alleles at 22 degrees C, but very poor interaction between GI (Cvi) and ZTL (Cvi) at higher temperature. In vivo, this would result in lower ZTL levels at high temperatures leading to longer periods in the Cvi background. Mismatched allelic combinations result in extremely strong or weak GI/ZTL interactions, indicating how the corresponding natural variants likely became fixed through epistatic selection. Additionally, molecular characterization of GI (Cvi) reveals a novel functional motif that can modulate the GI/ZTL interaction as well as nucleocytoplasmic partitioning. Taken together, these results identify a plausible temperature-dependent molecular mechanism, which contributes to the robustness of TC through natural variation in GI and ZTL alleles found on the Cape Verde Islands.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	ARABIDOPSIS-THALIANA ; POSTTRANSLATIONAL REGULATION ; EPISTATIC INTERACTIONS ; MOLECULAR-MECHANISMS ; PHOSPHORYLATION ; ZEITLUPE ; GIGANTEA ; RESPONSES ; GENES ; LOOP
WOS研究方向	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000513631100034
资助机构	National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM093285] ; Next-Generation BioGreen21 ProgramRural Development Administration (RDA) [PJ01327305] ; Rural Development Administration, Republic of KoreaRural Development Administration (RDA) ; Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries through Export Promotion Technology Development Program, Ministry of Agriculture, Food and Rural Affairs [117033-03-1-HD030]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21909]
专题	中科院植物分子生理学重点实验室
作者单位	1.Korea Natl Open Univ, Dept Agr Sci, Seoul 03087, South Korea 2.[Kim, Tae-Sung; Wang, Lei; Kim, Yeon Jeong; Somers, David E.] Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Kim, Tae-Sung,Wang, Lei,Kim, Yeon Jeong,et al. Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock[J]. PLANT PHYSIOLOGY,2020,182(2):1130-1141.
APA	Kim, Tae-Sung,Wang, Lei,Kim, Yeon Jeong,&Somers, David E..(2020).Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock.PLANT PHYSIOLOGY,182(2),1130-1141.
MLA	Kim, Tae-Sung,et al."Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock".PLANT PHYSIOLOGY 182.2(2020):1130-1141.