The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development

doi:10.1093/nar/gkaa129

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	The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development
	Zou, Meijuan ; Mu, Ying ; Chai, Xin 2; Ouyang, Min ; Yu, Long-Jiang ; Zhang, Lixin 1; Meurer, Joerg 3; Chi, Wei 2
刊名	NUCLEIC ACIDS RESEARCH
	2020
卷号	48 期号:6 页码:3195-3210
ISSN号	0305-1048
DOI	10.1093/nar/gkaa129
文献子类	Article
英文摘要	Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. The formation of methylated nucleotides is performed by a variety of RNA-methyltransferases. Chloroplasts of plant cells result from an endosymbiotic event and possess their own genome and ribosomes. However, enzymes responsible for rRNA methylation and the function of modified nucleotides in chloroplasts remain to be determined. Here, we identified an rRNA methyltransferase, CMAL (Chloroplast MraW-Like), in the Arabidopsis chloroplast and investigated its function. CMAL is the Arabidopsis ortholog of bacterial MraW/RsmH proteins and accounts to the N-4-methylation of C-1352 in chloroplast 16S rRNA, indicating that CMAL orthologs and this methyl-modification nucleotide is conserved between bacteria and the endosymbiont-derived eukaryotic organelle. The knockout of CMAL in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Interestingly, the loss of CMAL leads not only to defects in chloroplast function, but also to abnormal leaf and root development and overall plant morphology. Further investigation showed that CMAL is involved in the plant development probably by modulating auxin derived signaling pathways. This study uncovered the important role of 16S rRNA methylation mediated by CMAL in chloroplast ribosome biogenesis and plant development.
学科主题	Biochemistry & Molecular Biology
电子版国际标准刊号	1362-4962
出版地	OXFORD
WOS关键词	CHLOROPLAST RIBOSOME ; ESCHERICHIA-COLI ; AUXIN BIOSYNTHESIS ; MITOCHONDRIAL BIOGENESIS ; ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; MESSENGER-RNA ; TRANSLATION ; PROTEIN ; IDENTIFICATION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000525957500034
资助机构	Strategic Priority Research Program of CAS [XDB17030100] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009003-005] ; German Science FoundationGerman Research Foundation (DFG) [TRR 175]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21810]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China 4.Ludwig Maximilians Univ Munchen, Fac Biol, Plant Mol Biol, D-82152 Planegg Martinsried, Germany
推荐引用方式 GB/T 7714	Zou, Meijuan,Mu, Ying,Chai, Xin,et al. The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development[J]. NUCLEIC ACIDS RESEARCH,2020,48(6):3195-3210.
APA	Zou, Meijuan.,Mu, Ying.,Chai, Xin.,Ouyang, Min.,Yu, Long-Jiang.,...&Chi, Wei.(2020).The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.NUCLEIC ACIDS RESEARCH,48(6),3195-3210.
MLA	Zou, Meijuan,et al."The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development".NUCLEIC ACIDS RESEARCH 48.6(2020):3195-3210.