Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice

doi:10.1016/j.jplph.2020.153140

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	Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice
	Yang, Fan 3; Wang, Tai 2,3; Liu, Lingtong
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2020
卷号	248
关键词	K+ channel OsAKT1.2 Potassium Rice Pollen germination
ISSN号	0176-1617
DOI	10.1016/j.jplph.2020.153140
文献子类	Article
英文摘要	Potassium homeostasis is essential for pollen development and pollen-pistil interactions during the sexual reproduction of flowering plants. Here, we described the role of a Shaker K+ channel, OsAKT1.2, in rice pollen germination and growth. OsAKT1.2 is specifically expressed in the tricellular pollen, mature pollen grains and growing pollen tubes. Using CRISPR gene editing, we found that knockout lines did not differ from wildtype in vegetative growth, but showed decreased pollen germination rate both in the germination medium and in vivo. OsAKT1.2-GFP fusion protein was localized in the plasma membrane and enriched at the pollen tube tip. OsAKT1.2 could complement the yeast strain which is deficient in K+ intake. These findings suggest that OsAKT1.2 is associated with pollen germination and tube elongation in rice.
学科主题	Plant Sciences
电子版国际标准刊号	1618-1328
出版地	MUNICH
WOS关键词	AKT1 POTASSIUM CHANNEL ; TUBE GROWTH ; ARABIDOPSIS-THALIANA ; TRANSPORT ; EXPRESSION ; COMPONENTS ; CURRENTS ; MUTANTS ; ENCODES ; SYSTEM
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000530650500005
资助机构	Chinese Academy of SciencesChinese Academy of Sciences [XDA08010301]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21771]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yang, Fan,Wang, Tai,Liu, Lingtong. Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,248.
APA	Yang, Fan,Wang, Tai,&Liu, Lingtong.(2020).Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice.JOURNAL OF PLANT PHYSIOLOGY,248.
MLA	Yang, Fan,et al."Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice".JOURNAL OF PLANT PHYSIOLOGY 248(2020).