A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing

doi:10.1186/s12284-020-00425-0

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing
	Ge, Qiang 1,2; Tang, Yongyan ; Luo, Wei ; Zhang, Jingyu ; Chong, Kang 3; Xu, Yunyuan 3
刊名	RICE
	2020
卷号	13 期号:1
关键词	OsCYP20-2 Grain length OsSYF2 Spliceosome Alternative splicing
ISSN号	1939-8425
DOI	10.1186/s12284-020-00425-0
文献子类	Article
英文摘要	Background Grain size is one of the key agronomic traits that impact grain yield. Several regulatory pathways had been reported to participate in grain size determination via cell expansion or proliferation in rice. However, little is known about cyclophilin and spliceosome participation in grain shape regulation. Results Here, we identified OsCYP20-2, a cyclophilin that influences spliceosome assembly to determine grain length.oscyp20-2 t1, a knock out mutant ofOsCYP20-2caused by T-DNA insertion, produced shorter grains with deficient cell elongation. Through yeast two-hybrid screening and pull-down assays, OsSYF2, a pre-mRNA splicing factor, was identified as an interacting protein of OsCYP20-2. The phenotypes of transgenic lines indicated thatOsSYF2positively regulates grain length via its influence on cell expansion. Transcriptomic analysis showed thatOsSYF2controls the expression and pre-mRNA alternative splicing of genes involved in sugar metabolism. In addition, these two genes have similar effects on panicle architecture. Conclusions Taken together, OsSYF2, an interacting protein of OsCYP20-2, controls grain length and panicle architecture by regulating the alternative splicing of pre-mRNA involved in cell elongation and sugar metabolism.
学科主题	Agronomy
电子版国际标准刊号	1939-8433
出版地	NEW YORK
WOS关键词	NATURAL VARIATION ; GENE-EXPRESSION ; RICE ; PROTEIN ; ENCODES ; ARABIDOPSIS ; SIZE ; IDENTIFICATION ; WEIGHT ; YIELD
WOS研究方向	Agriculture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000571813300001
资助机构	National Key Research and Development Program [2016YFD0100901]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21579]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Agr Univ, Coll Agron, Zhengzhou 450046, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Ge, Qiang,Tang, Yongyan,Luo, Wei,et al. A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing[J]. RICE,2020,13(1).
APA	Ge, Qiang,Tang, Yongyan,Luo, Wei,Zhang, Jingyu,Chong, Kang,&Xu, Yunyuan.(2020).A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing.RICE,13(1).
MLA	Ge, Qiang,et al."A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing".RICE 13.1(2020).