Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation

doi:10.1093/pcp/pcaa118

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	Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation
	Yu, Yihe 4; Meng, Xiangxuan 4; Guo, Dalong 4; Yang, Shengdi 4; Zhang, Guohai 4; Liang, Zhenchang 1,3
刊名	PLANT AND CELL PHYSIOLOGY
	2020
卷号	61 期号:12 页码:2043-2054
关键词	ABA Degradation E3 ubiquitin ligase Fruit ripening Ubiquitination
ISSN号	0032-0781
DOI	10.1093/pcp/pcaa118
文献子类	Article
英文摘要	The plant U-box E3 ubiquitin ligase-mediated ubiquitin/26S proteasome degradation system plays a key role in plant growth and development. Previously identified as a member of the grape PUB gene family, PUB38 was shown to participate in the berry-ripening progress. Here, we demonstrate that the E3 ligase VlPUB38 mediates abscisic acid (ABA) synthesis via 26S proteasome degradation and its involvement in regulating fruit-ripening processes. Strawberry-overexpressing VlPUB38 lines displayed obvious inhibition of mature phenotype, and this was rescued by exogenous ABA treatment and MG132. Post-ABA treatment, expression levels of ABA response-related genes in VlPUB38-over-expressed Arabidopsis significantly exceeded controls. Strawberry and Arabidopsis ectopic expression assays suggest that VlPUB38 negatively regulates fruit ripening in an ABA-dependent manner. Moreover, VlPUB38 has ubiquitin ligase activity, which depends on the U-box-conserved domain. VlPUB38 interacts with abscisic-aldehyde oxidase (VlAAO), targeting VlAAO proteolysis via the 26S proteasome system. These results indicate that VlPUB38 negatively regulates grape fruit ripening by mediating the degradation of key factor VlAAO in the ABA synthesis pathway.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	ANTHOCYANIN BIOSYNTHESIS ; PLANT-RESPONSE ; ARABIDOPSIS ; ACID ; STRAWBERRY ; STRESS ; ROLES ; SINAT5 ; IDENTIFICATION ; INTERACT
WOS研究方向	Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000627419900005
资助机构	National Key Research and Development Program of China [2018YFD1000105] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31701893, 32072517] ; Key R&D and Promotion Projects in Henan Province [202102110043] ; Training Project for Young Backbone Teachers of Colleges and Universities of Henan Province [81]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21464]
专题	中科院北方资源植物重点实验室
作者单位	1.Henan Engn Technol Res Ctr Qual Regulat & Control, Luoyang 471000, Henan, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, CAS Key Lab Plant Resource, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 4.Henan Univ Sci & Technol, Coll Forestry, Luoyang 471000, Henan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Yihe,Meng, Xiangxuan,Guo, Dalong,et al. Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation[J]. PLANT AND CELL PHYSIOLOGY,2020,61(12):2043-2054.
APA	Yu, Yihe,Meng, Xiangxuan,Guo, Dalong,Yang, Shengdi,Zhang, Guohai,&Liang, Zhenchang.(2020).Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation.PLANT AND CELL PHYSIOLOGY,61(12),2043-2054.
MLA	Yu, Yihe,et al."Grapevine U-Box E3 Ubiquitin Ligase VlPUB38 Negatively Regulates Fruit Ripening by Facilitating Abscisic-Aldehyde Oxidase Degradation".PLANT AND CELL PHYSIOLOGY 61.12(2020):2043-2054.