Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes

doi:10.1007/s00299-018-2302-9

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes
	Zhang, Langlang 2; Cheng, Jun 3; Sun, Xiaoming 3; Zhao, Tingting ; Li, Mingjun 4; Wang, Qingfeng ; Li, Shaohua 3; Xin, Haiping
刊名	PLANT CELL REPORTS
	2018
卷号	37 期号:8 页码:1159-1172
关键词	VaWRKY14 WRKY IIa Transcriptome Drought Grapevine
ISSN号	0721-7714
DOI	10.1007/s00299-018-2302-9
文献子类	Article
英文摘要	Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes, including COR15A, COR15B, COR413, KIN2, and RD29A. The WRKY family is one of a largest transcription factors in plants, and it is a key component of multiple stress responses. In this study, the drought- and cold-induced WRKY family gene VaWRKY14 was isolated and characterized. Phylogenetic analysis indicated that VaWRKY14 belongs to the WRKY IIa subfamily, of which several members participate in biotic and abiotic stress responses in plants. Fluorescence observation from Arabidopsis mesophyll protoplasts transformed with the VaWRKY14::eGFP fusion vector suggested that VaWRKY14 was localized in the nucleus. The VaWRKY14 in yeast cells did not display any transcriptional activity. The expression of VaWRKY14 could be induced by exogenous phytohormones, including salicylic acid (SA) and abscisic acid (ABA). Overexpression of VaWRKY14 enhanced the drought tolerance of transgenic Arabidopsis. Compared with wild-type Arabidopsis, the VaWRKY14-OE lines exhibited higher water content and antioxidant enzyme activities in leaves after drought treatment. RNA sequencing analysis revealed that several stress-related genes, including COR15A, COR15B, COR413, KIN2, and RD29A, were upregulated in transgenic plants relative to their expression in wild-type Arabidopsis under normal conditions. Several genes (3 upregulated and 49 down-regulated) modulated by VaWRKY14 were also affected by drought stress in wild-type plants. These data suggest that VaWRKY14 responds to drought and cold stresses and that drought tolerance may be enhanced by regulating the expression of stress-related genes in Arabidopsis.
学科主题	Plant Sciences
电子版国际标准刊号	1432-203X
出版地	NEW YORK
WOS关键词	WRKY TRANSCRIPTION FACTORS ; ABSCISIC-ACID ; COLD STRESS ; FORTUNELLA-CRASSIFOLIA ; NEGATIVE REGULATORS ; DISEASE RESISTANCE ; SALICYLIC-ACID ; DNA-BINDING ; RNA-SEQ ; RICE
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000438935500007
资助机构	National Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31471857] ; Youth Innovation Promotion Association of CAS [2015281] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502] ; Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas (NWAFU) [CSBAA2016009]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20710]
专题	中科院北方资源植物重点实验室
作者单位	1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Langlang,Cheng, Jun,Sun, Xiaoming,et al. Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes[J]. PLANT CELL REPORTS,2018,37(8):1159-1172.
APA	Zhang, Langlang.,Cheng, Jun.,Sun, Xiaoming.,Zhao, Tingting.,Li, Mingjun.,...&Xin, Haiping.(2018).Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes.PLANT CELL REPORTS,37(8),1159-1172.
MLA	Zhang, Langlang,et al."Overexpression of VaWRKY14 increases drought tolerance in Arabidopsis by modulating the expression of stress-related genes".PLANT CELL REPORTS 37.8(2018):1159-1172.