A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm

doi:10.1093/jxb/ery290

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	A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm
	Tian, Lihong ; Xing, Yanping ; Fukuda, Masako 1; Li, Rong ; Kumamaru, Toshihiro 1; Qian, Dandan ; Dong, Xiangbai ; Qu, Le Qing
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2018
卷号	69 期号:21 页码:5029-5043
关键词	Disulfide bonds endoplasmic reticulum export glutelin rice endosperm sequence motif protein assembly
ISSN号	0022-0957
DOI	10.1093/jxb/ery290
文献子类	Article
英文摘要	Rice glutelins are initially synthesized as 57-kDa precursors at the endoplasmic reticulum (ER) and are ultimately transported into protein storage vacuoles. However, the sequence motifs that affect proglutelin folding, assembly, and their export from the ER remain poorly defined. In this study, we characterized a mutant with nine amino acids deleted in the GluA2 protein, which resulted in specific accumulation of the GluA precursor. The deleted amino acids constitute a well-conserved sequence (LVYIIQGRG) in glutelins and all residues in this motif are necessary for ER export of GluA2. Immunoelectron microscopy and stable transgenic analyses indicated that proglutelins with deletion of this motif misassembled and aggregated through non-native intermolecular disulfide bonds, and were deposited in ER-derived protein bodies (PB-Is), resulting in conversion of PB-Is into a new type of PB. These results indicate that the conserved motif is essential for proper assembly of proglutelin. The correct assembly of proglutelins is critical for their segregation from prolamins in the ER lumen, which is essential for enabling the export of proglutelin from the ER and for the proper formation of PB-Is. We also found that the interchain disulfide bond between acidic and basic subunits is not necessary for their assembly, but it is required for proglutelin folding.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	PROTEIN STORAGE VACUOLE ; DISULFIDE BOND FORMATION ; INTRACELLULAR-TRANSPORT ; STARCHY ENDOSPERM ; GOLGI-APPARATUS ; ARABIDOPSIS-THALIANA ; GLUTELIN PRECURSOR ; RAB5 PROTEINS ; EXPRESSION ; ISOMERASE
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000448163900005
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD 0100500] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300212, 31171368]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20507]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Kyushu Univ, Fac Agr, Fukuoka, Fukuoka 8128581, Japan
推荐引用方式 GB/T 7714	Tian, Lihong,Xing, Yanping,Fukuda, Masako,et al. A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(21):5029-5043.
APA	Tian, Lihong.,Xing, Yanping.,Fukuda, Masako.,Li, Rong.,Kumamaru, Toshihiro.,...&Qu, Le Qing.(2018).A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm.JOURNAL OF EXPERIMENTAL BOTANY,69(21),5029-5043.
MLA	Tian, Lihong,et al."A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm".JOURNAL OF EXPERIMENTAL BOTANY 69.21(2018):5029-5043.