A new method for fast extraction and determination of chlorophylls in natural water

doi:10.1515/znc-2017-0157

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	A new method for fast extraction and determination of chlorophylls in natural water
	Qiu, Nianwei ; Wang, Xiushun 3,4; Zhou, Feng 2
刊名	ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
	2018
卷号	73 期号:1-2 页码:77-86
关键词	acetone chlorophyll determination dimethyl sulfoxide (DMSO) high-speed refrigerated centrifugation (HSRC) water sample
ISSN号	0939-5075
DOI	10.1515/znc-2017-0157
文献子类	Article
英文摘要	Algae collection and chlorophyll extraction are two troublesome steps in the traditional methods used for the determination of chlorophyll concentration in natural water. A new method was established in this study for fast collection and extraction of chlorophyll. Based on our results, the optimum centrifugation condition for collecting algae was determined as: 5000 g for 15 min at 4 degrees C, and the optimum dilution ratio of dimethyl sulfoxide: 90% acetone was 1:4. The specific steps were as follows: the algae in water samples were collected by centrifugation at 5000 g at 4 degrees C for 15 min. The precipitated algae were suspended with 2 mL DMSO. Then the sample was transferred to a 15 mL centrifuge tube, and the tube was incubated at 65 degrees C for 1-2 h in the dark until the sample turned white. After cooling, the chlorophyll extract was diluted with 8 mL 90% acetone, before centrifugation at 5000 g for 5 min. The absorbance values of the supernatants at 750, 664, 647 and 630 nm were used for the calculation of chlorophyll concentrations by the trichromatic equations. This new method saved the filter cost, simplified the extraction process, improved the algae acquisition efficiency, and accelerated the chlorophyll extraction rate.
学科主题	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
电子版国际标准刊号	1865-7125
出版地	BERLIN
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PHYTOPLANKTON CHLOROPHYLL ; CAROTENOIDS ; ALGAE ; EQUATIONS ; SOLVENTS
语种	英语
出版者	WALTER DE GRUYTER GMBH
WOS记录号	WOS:000426309100009
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471884] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2015JL012, ZR2015DL009] ; Postgraduate Education Innovation Project of Shandong Province [SDYY16087] ; China's Post-doctoral Science FundChina Postdoctoral Science Foundation [2016M592158] ; Refined Experimental Project of Qufu Normal University [JP2014006]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20448]
专题	中科院光生物学重点实验室
作者单位	1.Nanjing Xiaozhuang Univ, Sch Food Sci, Nanjing 211171, Jiangsu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 4.Qufu Normal Univ, Sch Life Sci, Qufu, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Nianwei,Wang, Xiushun,Zhou, Feng. A new method for fast extraction and determination of chlorophylls in natural water[J]. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES,2018,73(1-2):77-86.
APA	Qiu, Nianwei,Wang, Xiushun,&Zhou, Feng.(2018).A new method for fast extraction and determination of chlorophylls in natural water.ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES,73(1-2),77-86.
MLA	Qiu, Nianwei,et al."A new method for fast extraction and determination of chlorophylls in natural water".ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES 73.1-2(2018):77-86.