Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains

doi:10.1016/j.molp.2018.04.003

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	Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains
	Xue, Yiqun 5; Xing, Jingjing 5; Wan, Yinglang ; Lv, Xueqin ; Fan, Lusheng 1; Zhang, Yongdeng 2; Song, Kai 5; Wang, Li ; Wang, Xiaohua ; Deng, Xin
刊名	MOLECULAR PLANT
	2018
卷号	11 期号:6 页码:846-859
关键词	phot1 VA-TIRFM spatiotemporal dynamics blue light signaling membrane microdomains
ISSN号	1674-2052
DOI	10.1016/j.molp.2018.04.003
文献子类	Article
英文摘要	Phototropin (phot)-mediated signaling initiated by blue light (BL) plays a critical role in optimizing photosynthetic light capture at the plasma membrane (PM) in plants. However, the mechanisms underlying the regulation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and stepwise photobleaching analysis of phot1-GFP proteins we demonstrated that in the dark phot1 proteins remain in an inactive state and mostly exist as monomers. Dimerization and the diffusion rate of phot1-GFP increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive phot1(D806N) -GFP but did enhance its dimerization, suggesting that phot1 dimerization is independent of phosphorylation. Forster resonance energy transfer-fluorescence lifetime imaging microscopy analysis revealed that the interaction between phot1-GFP and a marker of sterolrich lipid environments, AtRem1.3-mCherry, was enhanced with increased time of BL treatment. However, this BL-dependent interaction was not obvious in plants co-expressing phot1(D806N)-GFP and AtRem1.3-mCherry, indicating that BL facilitates the translocation of functional phot1-GFP into AtRem1.3-labeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated phot1-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane micro-domains act as organizing platforms essential for the proper function of activated phot1 at the PM.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1752-9867
出版地	CAMBRIDGE
WOS关键词	SUBCELLULAR-LOCALIZATION ; PROVIDES EVIDENCE ; MOLECULE ; DYNAMICS ; OLIGOMERIZATION ; DIMERIZATION ; PROTEINS ; STEP ; AUTOPHOSPHORYLATION ; PHOSPHORYLATION
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000434429000008
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530084, 31270224] ; Program of Introducing Talents of Discipline to Universities (111 project)Ministry of Education, China - 111 Project [B13007] ; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/R001499/1] Funding Source: UKRI
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20433]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA 3.Yale Univ, Sch Med, Dept Cell Biol, 333 Cedar St, New Haven, CT 06520 USA 4.Univ Bonn, Inst Cellular & Mol Bot, D-53115 Bonn, Germany 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 6.Christie, John M.] MRC, Inst Mol Cell & Syst Biol, Bower Bldg, Glasgow G12 8QQ, Lanark, Scotland 7.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Xue, Yiqun,Xing, Jingjing,Wan, Yinglang,et al. Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains[J]. MOLECULAR PLANT,2018,11(6):846-859.
APA	Xue, Yiqun.,Xing, Jingjing.,Wan, Yinglang.,Lv, Xueqin.,Fan, Lusheng.,...&Lin, Jinxing.(2018).Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains.MOLECULAR PLANT,11(6),846-859.
MLA	Xue, Yiqun,et al."Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains".MOLECULAR PLANT 11.6(2018):846-859.