A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato

doi:10.1111/mpp.12774

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato
	Zheng, Xueao ; Li, Xiaojing 2; Wang, Bingsen ; Cheng, Dong ; Li, Yanping ; Li, Wenhao ; Huang, Mengshu ; Tan, Xiaodan ; Zhao, Guozhen ; Song, Botao 1
刊名	MOLECULAR PLANT PATHOLOGY
	2019
卷号	20 期号:4 页码:547-561
关键词	type III effector Ralstonia solanacearum plant immunity potato calcium signalling
ISSN号	1464-6722
DOI	10.1111/mpp.12774
文献子类	Article
英文摘要	Both Solanum tuberosum and Ralstonia solanacearum phylotype IIB originated in South America and share a long-term co-evolutionary history. However, our knowledge of potato bacterial wilt pathogenesis is scarce as a result of the technical difficulties of potato plant manipulation. Thus, we established a multiple screening system (virulence screen of effector mutants in potato, growth inhibition of yeast and transient expression in Nicotiana benthamiana) of core type III effectors (T3Es) of a major potato pathovar of phylotype IIB, to provide more research perspectives and biological tools. Using this system, we identified four effectors contributing to virulence during potato infection, with two exhibiting multiple phenotypes in two other systems, including RipAB. Further study showed that RipAB is an unknown protein with a nuclear localization signal (NLS). Furthermore, we generated a ripAB complementation strain and transgenic ripAB-expressing potato plants, and subsequent virulence assays confirmed that R. solanacearum requires RipAB for full virulence. Compared with wild-type potato, transcriptomic analysis of transgenic ripAB-expressing potato plants showed a significant down-regulation of Ca2+ signalling-related genes in the enriched Plant-Pathogen Interaction (PPI) gene ontology (GO) term. We further verified that, during infection, RipAB is required for the down-regulation of four Ca2+ sensors, Stcml5, Stcml23, Stcml-cast and Stcdpk2, and a Ca2+ transporter, Stcngc1. Further evidence showed that the immune-associated reactive oxygen species (ROS) burst is attenuated in ripAB transgenic potato plants. In conclusion, a systematic screen of conserved R. solanacearum effectors revealed an important role for RipAB, which interferes with Ca2+-dependent gene expression to promote disease development in potato.
学科主题	Plant Sciences
电子版国际标准刊号	1364-3703
出版地	HOBOKEN
WOS关键词	PROGRAMMED CELL-DEATH ; DEPENDENT PROTEIN-KINASES ; III EFFECTORS ; SECRETION SYSTEM ; PLANT-PATHOGEN ; PHYTOPHTHORA-INFESTANS ; NICOTIANA-BENTHAMIANA ; BROWN-ROT ; IN-VIVO ; BACTERIAL
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000461896600007
资助机构	Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2013PY080] ; Initial Project Fund from Huazhong Agricultural University [12012] ; Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences) ; Chinese 1000 Talents Program ; Earmarked Fund for Modern Agro-Industry Technology Research System of China [CARS-09-P07]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19884]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Huazhong Agr Univ, Key Lab Potato Biol & Biotechnol, Minist Agr & Rural Affairs, Wuhan 430070, Hubei, Peoples R China 3.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Hubei, Peoples R China 4.Macho, Alberto P.] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Xueao,Li, Xiaojing,Wang, Bingsen,et al. A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato[J]. MOLECULAR PLANT PATHOLOGY,2019,20(4):547-561.
APA	Zheng, Xueao.,Li, Xiaojing.,Wang, Bingsen.,Cheng, Dong.,Li, Yanping.,...&Xie, Conghua.(2019).A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato.MOLECULAR PLANT PATHOLOGY,20(4),547-561.
MLA	Zheng, Xueao,et al."A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato".MOLECULAR PLANT PATHOLOGY 20.4(2019):547-561.