An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon

doi:10.1104/pp.18.00875

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon
	Wu, Hongyu ; Xue, Xiaodong ; Qin, Caihua ; Xu, Yi ; Guo, Yuyu ; Li, Xiang ; Lv, Wei ; Li, Qinxia ; Mao, Chuangxue ; Li, Luzhao
刊名	PLANT PHYSIOLOGY
	2019
卷号	180 期号:1 页码:56-65
ISSN号	0032-0889
DOI	10.1104/pp.18.00875
文献子类	Article
英文摘要	Transposon tagging is a powerful tool that has been widely applied in several species for insertional mutagenesis in plants. Several efforts have aimed to create transfer-DNA (T-DNA) insertional mutant populations in Brachypodium distachyon, a monocot plant used as a model system to study temperate cereals, but there has been a lack of research aimed at using transposon strategies. Here, we describe the application of a maize (Zea mays) Dissociation (Ds) transposon tagging system in B. distachyon. The 35S::AcTPase cassette and Ds element were constructed within the same T-DNA and transformed into B. distachyon plants. The Ds element was readily transposed to other chromosomes or to the same chromosome under the function of Activator (Ac) transposase. Through homologous chromosome synapsis, recombination, and segregation, the Ds element separated from the Ac element. We selected stable Ds-only plants using G418 and GFP assays and analyzed 241 T-0 lines, some of which were highly efficient at producing Ds-only progeny. Through thermal asymmetric interlaced PCR, we isolated 710 independent Ds flanking sequences from Ds-only plants. Furthermore, we identified a large collection of mutants with visible developmental phenotypes via this transposon tagging system. The system is relatively simple and rapid in comparison to traditional T-DNA insertion strategies, because once efficiency lines are obtained they can be reused to generate more lines from nontransposed plants without the use of time-consuming tissue culture steps.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	CARY
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; FUNCTIONAL GENOMICS ; MODEL ; ELEMENTS ; LINES ; MUTAGENESIS ; SELECTION ; BIOLOGY ; TRAP ; AC
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000466860800013
资助机构	Ministry of Agriculture and Rural Affairs of China [2016ZX08009-002] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2014CB138102] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570290] ; State Key Laboratory of Crop Biology [dxkt201705] ; Double Tops Project of Shandong Province
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19825]
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Hongyu,Xue, Xiaodong,Qin, Caihua,et al. An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon[J]. PLANT PHYSIOLOGY,2019,180(1):56-65.
APA	Wu, Hongyu.,Xue, Xiaodong.,Qin, Caihua.,Xu, Yi.,Guo, Yuyu.,...&An, Hailong.(2019).An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon.PLANT PHYSIOLOGY,180(1),56-65.
MLA	Wu, Hongyu,et al."An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon".PLANT PHYSIOLOGY 180.1(2019):56-65.